Functional photoacoustic micro-imaging of cerebral hemodynamic changes in single blood vessels after photo-induced brain stroke

Lun De Liao, You Yin Chen, Chin Teng Lin, Meng Lin Li

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Studying the functional hemodynamic roles of individual cerebral cortical arterioles in maintaining both the structure and function of cortical regions during and after brain stroke in small animals is an important issue. Recently, functional photoacoustic microscopy (fPAM) has been proved as a reliable imaging technique to probe the total hemoglobin concentration (HbT), cerebral blood volume (CBV) and hemoglobin oxygen saturation (SO2) in single cerebral blood vessels of rats. Here, we report the application of fPAM associated with electrophysiology recordings to investigating functional hemodynamic changes in single cortical arterioles of rats with electrical forepaw stimulation after photo-induced ischemic stroke. Because of the weak optical focusing nature of our fPAM system, photo-induced ischemic stroke targeting single cortical arterioles can be easily conducted with simple adaptation. Functional HbT, CBV and SO2 changes associated with the induced stroke in selected arterioles from the anterior cerebral artery system were imaged with 36 x 65-μm spatial resolution. Experimental results showed that after photo-occlusion of a single arteriole, the functional changes of nearby arterioles in cerebral cortex only can be observed immediately after the stroke. After a few minutes of stroke onset, there are no significant functional changes under the forepaw stimulation, suggesting that alternate blood flow routes are not actively recruited. The fPAM with electrophysiology recordings complements existing imaging techniques and has the potential to offer a favorable tool for explicitly studying cerebral hemodynamics in small animal models of photo-indcued ischemic stroke.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2013
StatePublished - 2013
EventPhotons Plus Ultrasound: Imaging and Sensing 2013 - San Francisco, CA, United States
Duration: 3 Feb 20135 Feb 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferencePhotons Plus Ultrasound: Imaging and Sensing 2013
Country/TerritoryUnited States
CitySan Francisco, CA


  • Cerebral hemodynamic response
  • Electrophysiology recording
  • Forepaw electrical stimulation
  • Ischemic stroke
  • Photoacoustic microscopy


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