Functional characterization of AIBp, a novel Aurora-A binding protein in centrosome structure and spindle formation

Ann Shung Lieu, Tai Shan Cheng, Chia Hua Chou, Chia Hung Wu, Chia Yi Hsu, Chi Ying F. Huang, Li Kwan Chang, Joon Khim Loh, Chung Shing Chang, Ching Mei Hsu, Shen Long Howng, Yi Ren Hong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Aurora-A is involved in chromosome alignment, centrosome maturation, mitotic spindle assembly and regards to an oncogene. Aurora-A is also known to bind to several other proteins affecting its up-regulation or down-regulation and localization. However, how these different binding signals work together to regulate Aurora-A is not properly known. To explore more Aurora-A interacting proteins, the low-copy yeast two-hybrid screening using Aurora-A as bait protein was performed. One novel gene, AIBp, was demonstrated to associate with Aurora-A by the yeast two-hybrid method and in vitro GST pull-down assay. Molecular characterization showed that AIBp possessed a binding site at the C-terminal with Aurora-A (kinase domain). Interestingly, AIBp also interacts with hNinein at the N-terminal, which overlaps with a previously reported hNinein and GSK3ß binding site. Using a kinase assay, AIBp interacts with the Aurora-A kinase domain functions as a positive regulator, whereas AIBp binding to hNinein appears to block the phosphorylation of hNinein by both Aurora-A and GSK3ß. siRNA-mediated elimination of AIBp from HeLa cells, results in a doughnut-like shape, asymmetrical spindle pole and multiple spindle pole formation. We also demonstrated that both AIBp and Aurora-A are co-overexpressed in various brain tumors. These studies demonstrate that AIBp may not only be required for the dynamic movement of Aurora-A at the centrosomes and spindle apparatus during the cell cycle, but may also be important during brain tumorigenesis.

Original languageEnglish
Pages (from-to)429-436
Number of pages8
JournalInternational journal of oncology
Volume37
Issue number2
DOIs
StatePublished - Aug 2010

Keywords

  • AIBp
  • Aurora-A
  • Brain tumor
  • Centrosome
  • Ninein
  • Spindle

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