FRET-based dual channel fluorescent probe for detecting endogenous/exogenous H 2 O 2 /H 2 S formation through multicolor images

Nithya Velusamy, Natesan Thirumalaivasan, Kondapa Naidu Bobba, Arup Podder, Shu-Pao Wu*, Sankarprasad Bhuniya

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

We have developed a FRET-based fluorescent probe (PHS1) as a combination of two different fluorophores (coumarin and naphthalimide); which can detect both exogenous and endogenous H 2 S and H 2 O 2 in live cells through multicolor images. The precise overlap between UV-absorption of naphthalimide and the emission band of coumarin in probe PHS1 allows the acquisition of the self-calibrated information of dual analytes through FRET-based imaging. The UV–Vis absorption (λ abs 390 nm) and fluorescence emission (λ em 460 nm) of probe PHS1 in the presence of H 2 O 2 are increased ∽35- fold and ∽15-fold respectively. It also allows the estimation of the levels of H 2 S through enhancement of emission intensity at 550 nm. The probe PHS1 exhibits high stability against various analytes, including various pH (4–9.5). The cell viability assay data indicate that the probe is not harmful to the cancer cells. The nontoxic nature of the probe PHS1 encourages application for cancer cell labeling. The probe PHS1 can detect the level of endogenous H 2 O 2 , H 2 S, and H 2 O 2 /H 2 S in cancer cells through blue, green and FRET-based green channel imaging. PHS1 is a unique probe, has potential application for diagnosing cancer by providing information on the level of dual analytes (H 2 S, H 2 O 2 ) in cancer cells.

Original languageEnglish
Pages (from-to)99-106
Number of pages8
JournalJournal of Photochemistry and Photobiology B: Biology
Volume191
DOIs
StatePublished - Feb 2019

Keywords

  • Coumarin
  • Endogenous H O
  • Endogenous H S
  • FRET- based fluorescent probe
  • Naphthalimide

Fingerprint

Dive into the research topics of 'FRET-based dual channel fluorescent probe for detecting endogenous/exogenous H 2 O 2 /H 2 S formation through multicolor images'. Together they form a unique fingerprint.

Cite this