TY - JOUR
T1 - FRET-based dual channel fluorescent probe for detecting endogenous/exogenous H 2 O 2 /H 2 S formation through multicolor images
AU - Velusamy, Nithya
AU - Thirumalaivasan, Natesan
AU - Bobba, Kondapa Naidu
AU - Podder, Arup
AU - Wu, Shu-Pao
AU - Bhuniya, Sankarprasad
N1 - Publisher Copyright:
© 2018
PY - 2019/2
Y1 - 2019/2
N2 - We have developed a FRET-based fluorescent probe (PHS1) as a combination of two different fluorophores (coumarin and naphthalimide); which can detect both exogenous and endogenous H 2 S and H 2 O 2 in live cells through multicolor images. The precise overlap between UV-absorption of naphthalimide and the emission band of coumarin in probe PHS1 allows the acquisition of the self-calibrated information of dual analytes through FRET-based imaging. The UV–Vis absorption (λ abs 390 nm) and fluorescence emission (λ em 460 nm) of probe PHS1 in the presence of H 2 O 2 are increased ∽35- fold and ∽15-fold respectively. It also allows the estimation of the levels of H 2 S through enhancement of emission intensity at 550 nm. The probe PHS1 exhibits high stability against various analytes, including various pH (4–9.5). The cell viability assay data indicate that the probe is not harmful to the cancer cells. The nontoxic nature of the probe PHS1 encourages application for cancer cell labeling. The probe PHS1 can detect the level of endogenous H 2 O 2 , H 2 S, and H 2 O 2 /H 2 S in cancer cells through blue, green and FRET-based green channel imaging. PHS1 is a unique probe, has potential application for diagnosing cancer by providing information on the level of dual analytes (H 2 S, H 2 O 2 ) in cancer cells.
AB - We have developed a FRET-based fluorescent probe (PHS1) as a combination of two different fluorophores (coumarin and naphthalimide); which can detect both exogenous and endogenous H 2 S and H 2 O 2 in live cells through multicolor images. The precise overlap between UV-absorption of naphthalimide and the emission band of coumarin in probe PHS1 allows the acquisition of the self-calibrated information of dual analytes through FRET-based imaging. The UV–Vis absorption (λ abs 390 nm) and fluorescence emission (λ em 460 nm) of probe PHS1 in the presence of H 2 O 2 are increased ∽35- fold and ∽15-fold respectively. It also allows the estimation of the levels of H 2 S through enhancement of emission intensity at 550 nm. The probe PHS1 exhibits high stability against various analytes, including various pH (4–9.5). The cell viability assay data indicate that the probe is not harmful to the cancer cells. The nontoxic nature of the probe PHS1 encourages application for cancer cell labeling. The probe PHS1 can detect the level of endogenous H 2 O 2 , H 2 S, and H 2 O 2 /H 2 S in cancer cells through blue, green and FRET-based green channel imaging. PHS1 is a unique probe, has potential application for diagnosing cancer by providing information on the level of dual analytes (H 2 S, H 2 O 2 ) in cancer cells.
KW - Coumarin
KW - Endogenous H O
KW - Endogenous H S
KW - FRET- based fluorescent probe
KW - Naphthalimide
UR - http://www.scopus.com/inward/record.url?scp=85059181985&partnerID=8YFLogxK
U2 - 10.1016/j.jphotobiol.2018.12.016
DO - 10.1016/j.jphotobiol.2018.12.016
M3 - Article
C2 - 30597284
AN - SCOPUS:85059181985
SN - 1011-1344
VL - 191
SP - 99
EP - 106
JO - Journal of Photochemistry and Photobiology B: Biology
JF - Journal of Photochemistry and Photobiology B: Biology
ER -
