Fluorescence lifetime-resolved imaging

Yi-Chun Chen, Robert M. Clegg

Research output: Contribution to journalReview articlepeer-review

38 Scopus citations


This is a short account of fluorescence lifetime-resolved imaging, in order to acquaint readers who are not experts with the basic methods for measuring lifetime-resolved signals throughout an image. We present the early FLI (fluorescence lifetime imaging) history, review shortly the instrumentation and experimental design, discuss briefly the fundamentals of the measured fluorescence response, and introduce the basic measurement methodologies. We also emphasize the complex nature of the fluorescence response in FLI signals, and introduce certain analysis methods that are appropriate and informative for complex fluorescence decays. The advantages of model independent analyses are discussed and examples given.

Original languageEnglish
Pages (from-to)143-155
Number of pages13
JournalPhotosynthesis Research
Issue number2
StatePublished - 1 Nov 2009


  • Fluorescence lifetime-resolved imaging
  • Fluorescence lifetime-resolved imaging microscopy
  • Frequency domain
  • Microscopy
  • Model independent analysis
  • Spectral FLIM
  • Time-domain


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