Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

Jianhong Ge, Yixin Wang, Cuifang Kuang, Shin Shian Lee, Nirmal Mazumder, Fu Jen Kao*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XIII
DOIs
StatePublished - 2013
EventMultiphoton Microscopy in the Biomedical Sciences XIII - San Francisco, CA, United States
Duration: 3 Feb 20135 Feb 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8588
ISSN (Print)1605-7422

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XIII
Country/TerritoryUnited States
CitySan Francisco, CA
Period3/02/135/02/13

Keywords

  • Diode laser
  • Fluorescence lifetime imaging
  • Stimulated emission

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