Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission

Jianhong Ge*, Cuifang Kuang, Shin Shian Lee, Fu Jen Kao

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance allsemiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.

Original languageEnglish
Pages (from-to)28216-28221
Number of pages6
JournalOptics Express
Issue number27
StatePublished - 17 Dec 2012


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