FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1)

Chun Yu Chen; Ning Sheng Lai; Jaw Ji Yang; Hsien lu Huang; Wei Chuan Hung; Chin Li; Ta Hsien Lin; Hsien bin Huang

doi:10.1016/j.bbrc.2009.11.123

FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1)

Chun Yu Chen, Ning Sheng Lai, Jaw Ji Yang, Hsien lu Huang, Wei Chuan Hung, Chin Li, Ta Hsien Lin^*, Hsien bin Huang

^*Corresponding author for this work

Department of Biochemistry

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

In this report, we identified the novel protein heart protein phosphatase 1-binding protein (Hepp1), encoded by FLJ23654. Hepp1 associated with protein phosphatase 1 (PP1) by yeast two-hybrid, GST pull-down, co-immunoprecipitation, and far Western blotting assays. Northern blot analysis revealed that Hepp1 mRNA was only expressed in human heart and testis. Recombinant Hepp1 slightly enhanced the enzymatic activity of PP1 and antagonized the ability of phospho-inhibitor-1 or inhibitor-2 to inhibit PP1. Hepp1 protein in human heart tissues was detected by Western blot analysis. Together, our data suggest that Hepp1 can play a role in cardiac functions by working in concert with PP1.

Original language	English
Pages (from-to)	698-702
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	391
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2009.11.123
State	Published - 1 Jan 2010

Keywords

Cardiac functions
FLJ23654
Inhibitor-1
Protein phosphatase 1
Yeast two-hybrid screen

Access to Document

10.1016/j.bbrc.2009.11.123

Cite this

@article{d6aed6a616374fe69d691381ae776c40,

title = "FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1)",

abstract = "In this report, we identified the novel protein heart protein phosphatase 1-binding protein (Hepp1), encoded by FLJ23654. Hepp1 associated with protein phosphatase 1 (PP1) by yeast two-hybrid, GST pull-down, co-immunoprecipitation, and far Western blotting assays. Northern blot analysis revealed that Hepp1 mRNA was only expressed in human heart and testis. Recombinant Hepp1 slightly enhanced the enzymatic activity of PP1 and antagonized the ability of phospho-inhibitor-1 or inhibitor-2 to inhibit PP1. Hepp1 protein in human heart tissues was detected by Western blot analysis. Together, our data suggest that Hepp1 can play a role in cardiac functions by working in concert with PP1.",

keywords = "Cardiac functions, FLJ23654, Inhibitor-1, Protein phosphatase 1, Yeast two-hybrid screen",

author = "Chen, {Chun Yu} and Lai, {Ning Sheng} and Yang, {Jaw Ji} and Huang, {Hsien lu} and Hung, {Wei Chuan} and Chin Li and Lin, {Ta Hsien} and Huang, {Hsien bin}",

note = "Funding Information: This work was supported by grants from the National Science Council, ROC (NSC 94-2320-B-194-005 and NSC 95-2320-B-194-005) and the Taipei Veterans General Hospital, Taiwan, ROC (VGH 92-375, VGH 93-359 and VGH 94-370). ",

year = "2010",

month = jan,

day = "1",

doi = "10.1016/j.bbrc.2009.11.123",

language = "English",

volume = "391",

pages = "698--702",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1)

AU - Chen, Chun Yu

AU - Lai, Ning Sheng

AU - Yang, Jaw Ji

AU - Huang, Hsien lu

AU - Hung, Wei Chuan

AU - Li, Chin

AU - Lin, Ta Hsien

AU - Huang, Hsien bin

N1 - Funding Information: This work was supported by grants from the National Science Council, ROC (NSC 94-2320-B-194-005 and NSC 95-2320-B-194-005) and the Taipei Veterans General Hospital, Taiwan, ROC (VGH 92-375, VGH 93-359 and VGH 94-370).

PY - 2010/1/1

Y1 - 2010/1/1

N2 - In this report, we identified the novel protein heart protein phosphatase 1-binding protein (Hepp1), encoded by FLJ23654. Hepp1 associated with protein phosphatase 1 (PP1) by yeast two-hybrid, GST pull-down, co-immunoprecipitation, and far Western blotting assays. Northern blot analysis revealed that Hepp1 mRNA was only expressed in human heart and testis. Recombinant Hepp1 slightly enhanced the enzymatic activity of PP1 and antagonized the ability of phospho-inhibitor-1 or inhibitor-2 to inhibit PP1. Hepp1 protein in human heart tissues was detected by Western blot analysis. Together, our data suggest that Hepp1 can play a role in cardiac functions by working in concert with PP1.

AB - In this report, we identified the novel protein heart protein phosphatase 1-binding protein (Hepp1), encoded by FLJ23654. Hepp1 associated with protein phosphatase 1 (PP1) by yeast two-hybrid, GST pull-down, co-immunoprecipitation, and far Western blotting assays. Northern blot analysis revealed that Hepp1 mRNA was only expressed in human heart and testis. Recombinant Hepp1 slightly enhanced the enzymatic activity of PP1 and antagonized the ability of phospho-inhibitor-1 or inhibitor-2 to inhibit PP1. Hepp1 protein in human heart tissues was detected by Western blot analysis. Together, our data suggest that Hepp1 can play a role in cardiac functions by working in concert with PP1.

KW - Cardiac functions

KW - FLJ23654

KW - Inhibitor-1

KW - Protein phosphatase 1

KW - Yeast two-hybrid screen

UR - http://www.scopus.com/inward/record.url?scp=72949095116&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.11.123

DO - 10.1016/j.bbrc.2009.11.123

M3 - Article

C2 - 19945436

AN - SCOPUS:72949095116

SN - 0006-291X

VL - 391

SP - 698

EP - 702

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1)

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this