FLJ23654 encodes a heart protein phosphatase 1-binding protein (Hepp1)

Chun Yu Chen, Ning Sheng Lai, Jaw Ji Yang, Hsien lu Huang, Wei Chuan Hung, Chin Li, Ta Hsien Lin*, Hsien bin Huang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


In this report, we identified the novel protein heart protein phosphatase 1-binding protein (Hepp1), encoded by FLJ23654. Hepp1 associated with protein phosphatase 1 (PP1) by yeast two-hybrid, GST pull-down, co-immunoprecipitation, and far Western blotting assays. Northern blot analysis revealed that Hepp1 mRNA was only expressed in human heart and testis. Recombinant Hepp1 slightly enhanced the enzymatic activity of PP1 and antagonized the ability of phospho-inhibitor-1 or inhibitor-2 to inhibit PP1. Hepp1 protein in human heart tissues was detected by Western blot analysis. Together, our data suggest that Hepp1 can play a role in cardiac functions by working in concert with PP1.

Original languageEnglish
Pages (from-to)698-702
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - 1 Jan 2010


  • Cardiac functions
  • FLJ23654
  • Inhibitor-1
  • Protein phosphatase 1
  • Yeast two-hybrid screen


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