Fibronectin Suppresses Lipopolysaccharide-Induced Liver Damage and Promotes the Cytoprotection Abilities of Hepatocyte-like Cells Derived From Human Bone Marrow Mesenchymal Stem Cells

C. L. Kao, H. T. Lin, Y. W. Chen, Y. C. Chen, F. T. Tsai, Y. L. Chang, S. H. Chiou, D. C. Sheu, H. H. Ku*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

We isolated mesenchymal stem cells (MSCs) from adult human bone marrow. By using reverse-transcription polymerase chain reactions, we confirmed that MSCs possessed the potential to differentiate into hepatocyte-like cells (MSC-HLCs) with the expression of hepatocyte-specific marker genes. We further observed that fibronectin (FN) treatment significantly inhibited lipopolysaccharide (LPS)-induced apoptotic activities in FN-treated MSC-HLCs, as detected by caspase 3 enzyme-linked immunosorbent (ELISA) and terminal dUTP nick-end labeling (TUNEL) assays (P < .05). The FN-treated MSC-HLCs were transplanted into SCID mice with or without LPS injection. This study demonstrated that FN treatment improved liver function repair and survival rates among LPS-treated SCID mice.

Original languageEnglish
Pages (from-to)3444-3445
Number of pages2
JournalTransplantation Proceedings
Volume39
Issue number10
DOIs
StatePublished - Dec 2007

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