Fast determination of three-dimensional fibril orientation of type-I collagen via macroscopic chirality

Guan Yu Zhuo, Mei Yu Chen, Chao Yuan Yeh, Chin Lin Guo, Fu Jen Kao

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17 Scopus citations

Abstract

Polarization-resolved second harmonic generation (SHG) microscopy is appealing for studying structural proteins and well-organized biophotonic nanostructures, due to its highly sensitized structural specificity. In recent years, it has been used to investigate the chiroptical effect, particularly SHG circular dichroism (SHG-CD) in biological tissues. Although SHG-CD attributed to macromolecular structures has been demonstrated, the corresponding quantitative analysis and interpretation on how SHG correlates with second-order susceptibility χ(2) under circularly polarized excitations remains unclear. In this study, we demonstrate a method based on macroscopic chirality to elucidate the correlation between SHG-CD and the orientation angle of the molecular structure. By exploiting this approach, three-dimensional (3D) molecular orientation of type-I collagen is revealed with only two cross polarized SHG images (i.e., interactions of left and right circular polarizations) without acquiring an image stack of varying polarization.

Original languageEnglish
Article number023702
JournalApplied Physics Letters
Volume110
Issue number2
DOIs
StatePublished - 9 Jan 2017

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