TY - JOUR
T1 - Fabrication and characterization of polymethylmethacrylate (PMMA) thin film by plasma polymerization used for cell culture
AU - Li, Chuan
AU - Hsieh, J. H.
AU - Hu, W. W.
AU - Lin, Y. H.
N1 - Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2014/11/25
Y1 - 2014/11/25
N2 - Poly-methyl-methacrylate thin films were deposited by plasma polymerization under different radio-frequency discharge powers, monomer partial pressures, and deposition times. Surface profiler was used to evaluate the thickness and deposition rates of deposited films; Fourier transform infrared spectroscopy for the microstructures; X-ray photoelectron spectroscopy for the surface chemical compositions and atomic force microscope for the surface morphology. In addition to the as-deposited PMMA films, some selected films were further treated by oxygen to modify their surfaces. After thorough material characterizations, the films were used as substrates to culture human hepatocytes for testing their biocompatibility. Results of the cell culture were assessed by trypsin cell count, ELISA and MTT assay. It is found that the human hepatocytes can proliferate and perform urea synthesis well on the deposited poly-methyl-methacrylate thin films with or without oxygen treatment.
AB - Poly-methyl-methacrylate thin films were deposited by plasma polymerization under different radio-frequency discharge powers, monomer partial pressures, and deposition times. Surface profiler was used to evaluate the thickness and deposition rates of deposited films; Fourier transform infrared spectroscopy for the microstructures; X-ray photoelectron spectroscopy for the surface chemical compositions and atomic force microscope for the surface morphology. In addition to the as-deposited PMMA films, some selected films were further treated by oxygen to modify their surfaces. After thorough material characterizations, the films were used as substrates to culture human hepatocytes for testing their biocompatibility. Results of the cell culture were assessed by trypsin cell count, ELISA and MTT assay. It is found that the human hepatocytes can proliferate and perform urea synthesis well on the deposited poly-methyl-methacrylate thin films with or without oxygen treatment.
KW - Atomic force microscope
KW - Fourier transform infrared spectroscopy
KW - Human hepatocyte
KW - Plasma polymerization
KW - Polymethylmethacrylate
UR - http://www.scopus.com/inward/record.url?scp=84919963333&partnerID=8YFLogxK
U2 - 10.1016/j.surfcoat.2014.07.066
DO - 10.1016/j.surfcoat.2014.07.066
M3 - Article
AN - SCOPUS:84919963333
SN - 0257-8972
VL - 259
SP - 20
EP - 26
JO - Surface and Coatings Technology
JF - Surface and Coatings Technology
IS - PA
ER -