Evolutionary conservation of DNA-contact residues in DNA-binding domains

Yao L. Chang, Huai Kuang Tsai, Cheng Y. Kao, Yung Chian Chen, Jinn-Moon Yang*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

DNA-binding proteins are of utmost importance to gene regulation. The identification of DNA-binding domains is useful for understanding the regulation mechanisms of DNA-binding proteins. In this study, we proposed a method to determine whether a domain or a protein can has DNA binding capability by considering evolutionary conservation of DNA-binding residues. Our method achieves high precision and recall for 66 families of DNA-binding domains, with a false positive rate less than 5% for 250 non-DNA-binding proteins. In addition, experimental results show that our method is able to identify the different DNA-binding behaviors of proteins in the same SCOP family based on the use of evolutionary conservation of DNA-contact residues.

Original languageEnglish
Title of host publicationProceedings - 2nd International Multi-Symposiums on Computer and Computational Sciences, IMSCCS'07
Pages9-16
Number of pages8
DOIs
StatePublished - 2007
Event2nd International Multi-Symposiums on Computer and Computational Sciences 2007, IMSCCS'07 - Iowa City, IA, United States
Duration: 13 Aug 200715 Aug 2007

Publication series

NameProceedings - 2nd International Multi-Symposiums on Computer and Computational Sciences, IMSCCS'07

Conference

Conference2nd International Multi-Symposiums on Computer and Computational Sciences 2007, IMSCCS'07
Country/TerritoryUnited States
CityIowa City, IA
Period13/08/0715/08/07

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