Evolutional Characterization of Photochemically Induced Stroke in Rats: a Multimodality Imaging and Molecular Biological Study

Nai Wei Liu, Chien Chih Ke, Yonghua Zhao*, Yi An Chen, Kim Chuan Chan, David Tat Wei Tan, Jhih Shian Lee, You Yin Chen, Tun Wei Hsu, Ya Ju Hsieh, Chi Wei Chang, Bang Hung Yang, Wen Sheng Huang, Ren Shyan Liu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

37 Scopus citations


Photochemically induced cerebral ischemia is an easy-manipulated, reproducible, relatively noninvasive, and lesion controllable model for translational study of ischemic stroke. In order to longitudinally investigate the characterization of the model, magnetic resonance imaging, 18F-2-deoxy-glucose positron emission tomography, fluorescence, and bioluminescence imaging system were performed in correlation with triphenyl tetrazolium chloride (TTC), hematoxylin-eosin staining, and immunohistochemistry examinations of glial fibrillary acidic protein, CD68, NeuN, von willebrand factor, and α-smooth muscle actin in the infarct zone. The results suggested that the number of inflammatory cells, astrocytes, and neovascularization significantly elevated in peri-infarct region from day 7 and a belt of macrophage/microglial and astrocytes was formed surrounding infarct lesion at day 14. Both vasogenic and cytotoxic edema, as well as blood brain-barrier leakage, occurred since day 1 after stroke induction and gradually attenuated with time. Numerous cells other than neuronal cells infiltrated into infarct lesion, which resulted in no visible TTC negative regional existence at day 14. Furthermore, recovery of cerebral blood flow and glucose utilization in peri-infarct zone were noted and more remarkably than that in infarct core following the stroke progression. In conclusion, these characterizations may be highly beneficial to the development of therapeutic strategies for ischemic stroke.

Original languageEnglish
Pages (from-to)244-256
Number of pages13
JournalTranslational Stroke Research
Issue number3
StatePublished - 1 Jun 2017


  • Multimodality imaging
  • Photothrombotic stroke


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