Evaluation of Wound Healing Effect on Skin-Defect Nude Mice by Using Human Dermis-Derived Mesenchyaml Stem Cells

C. K. Perng; H. H. Ku; S. H. Chiou; I. L. Chen; F. T. Tsai; Y. P. Yang; K. Y. Chang; C. L. Kao

doi:10.1016/j.transproceed.2006.08.146

Evaluation of Wound Healing Effect on Skin-Defect Nude Mice by Using Human Dermis-Derived Mesenchyaml Stem Cells

C. K. Perng, H. H. Ku, S. H. Chiou, I. L. Chen, F. T. Tsai, Y. P. Yang, K. Y. Chang, C. L. Kao^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We report that human dermis-derived mesenchymal stem cells (hDMSCs) possess differentiation potential of epidermis facilitating wound healing in skin-defect nude mice in combination with the treatment using gelatin/thermosensitive poly N-isopropylacrylamide (pNIPAAm)/polypropylene (PP). The results showed that the rate of cell growth and wound recovery in the hDMSC and gelatin/pNIPAAm/PP-treated group was significantly greater than those in the gelatin/pNIPAAm/PP-treated only group (P < .01). The reepithelialization marker of human pan-cytokeratin was also significantly increased on days 14 and day 21 in the wound site of hDMSCs and gelatin/pNIPAAm/PP-treated group. Furthermore, the stem cell marker of human CD13 gradually decreased during the period of wound healing. In sum, this novel method provided a transferring system for stem cell therapy, maintaining its temperature-sensitive property of easy peeling by lower temperature treatment.

Original language	English
Pages (from-to)	3086-3087
Number of pages	2
Journal	Transplantation Proceedings
Volume	38
Issue number	9
DOIs	https://doi.org/10.1016/j.transproceed.2006.08.146
State	Published - Nov 2006

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@article{5fe5f54a6e7a4b5daf7c9858a1ebc985,

title = "Evaluation of Wound Healing Effect on Skin-Defect Nude Mice by Using Human Dermis-Derived Mesenchyaml Stem Cells",

abstract = "We report that human dermis-derived mesenchymal stem cells (hDMSCs) possess differentiation potential of epidermis facilitating wound healing in skin-defect nude mice in combination with the treatment using gelatin/thermosensitive poly N-isopropylacrylamide (pNIPAAm)/polypropylene (PP). The results showed that the rate of cell growth and wound recovery in the hDMSC and gelatin/pNIPAAm/PP-treated group was significantly greater than those in the gelatin/pNIPAAm/PP-treated only group (P < .01). The reepithelialization marker of human pan-cytokeratin was also significantly increased on days 14 and day 21 in the wound site of hDMSCs and gelatin/pNIPAAm/PP-treated group. Furthermore, the stem cell marker of human CD13 gradually decreased during the period of wound healing. In sum, this novel method provided a transferring system for stem cell therapy, maintaining its temperature-sensitive property of easy peeling by lower temperature treatment.",

author = "Perng, {C. K.} and Ku, {H. H.} and Chiou, {S. H.} and Chen, {I. L.} and Tsai, {F. T.} and Yang, {Y. P.} and Chang, {K. Y.} and Kao, {C. L.}",

note = "Funding Information: Supported by Taipei Veterans General Hospital and Ministry of Education—Aim for the Top University Plan. ",

year = "2006",

month = nov,

doi = "10.1016/j.transproceed.2006.08.146",

language = "English",

volume = "38",

pages = "3086--3087",

journal = "Transplantation Proceedings",

issn = "0041-1345",

publisher = "Elsevier USA",

number = "9",

}

TY - JOUR

T1 - Evaluation of Wound Healing Effect on Skin-Defect Nude Mice by Using Human Dermis-Derived Mesenchyaml Stem Cells

AU - Perng, C. K.

AU - Ku, H. H.

AU - Chiou, S. H.

AU - Chen, I. L.

AU - Tsai, F. T.

AU - Yang, Y. P.

AU - Chang, K. Y.

AU - Kao, C. L.

N1 - Funding Information: Supported by Taipei Veterans General Hospital and Ministry of Education—Aim for the Top University Plan.

PY - 2006/11

Y1 - 2006/11

N2 - We report that human dermis-derived mesenchymal stem cells (hDMSCs) possess differentiation potential of epidermis facilitating wound healing in skin-defect nude mice in combination with the treatment using gelatin/thermosensitive poly N-isopropylacrylamide (pNIPAAm)/polypropylene (PP). The results showed that the rate of cell growth and wound recovery in the hDMSC and gelatin/pNIPAAm/PP-treated group was significantly greater than those in the gelatin/pNIPAAm/PP-treated only group (P < .01). The reepithelialization marker of human pan-cytokeratin was also significantly increased on days 14 and day 21 in the wound site of hDMSCs and gelatin/pNIPAAm/PP-treated group. Furthermore, the stem cell marker of human CD13 gradually decreased during the period of wound healing. In sum, this novel method provided a transferring system for stem cell therapy, maintaining its temperature-sensitive property of easy peeling by lower temperature treatment.

AB - We report that human dermis-derived mesenchymal stem cells (hDMSCs) possess differentiation potential of epidermis facilitating wound healing in skin-defect nude mice in combination with the treatment using gelatin/thermosensitive poly N-isopropylacrylamide (pNIPAAm)/polypropylene (PP). The results showed that the rate of cell growth and wound recovery in the hDMSC and gelatin/pNIPAAm/PP-treated group was significantly greater than those in the gelatin/pNIPAAm/PP-treated only group (P < .01). The reepithelialization marker of human pan-cytokeratin was also significantly increased on days 14 and day 21 in the wound site of hDMSCs and gelatin/pNIPAAm/PP-treated group. Furthermore, the stem cell marker of human CD13 gradually decreased during the period of wound healing. In sum, this novel method provided a transferring system for stem cell therapy, maintaining its temperature-sensitive property of easy peeling by lower temperature treatment.

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U2 - 10.1016/j.transproceed.2006.08.146

DO - 10.1016/j.transproceed.2006.08.146

M3 - Article

C2 - 17112905

AN - SCOPUS:33750935452

SN - 0041-1345

VL - 38

SP - 3086

EP - 3087

JO - Transplantation Proceedings

JF - Transplantation Proceedings

IS - 9

ER -

Evaluation of Wound Healing Effect on Skin-Defect Nude Mice by Using Human Dermis-Derived Mesenchyaml Stem Cells

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