ESIPT triggered swift determination of cysteine in HeLa cell line during redox imbalance

Kathiresan Anusuyadevi; Shu-Pao Wu; Sivan Velmathi

doi:10.1016/j.jphotochem.2020.112875

ESIPT triggered swift determination of cysteine in HeLa cell line during redox imbalance

Kathiresan Anusuyadevi, Shu-Pao Wu, Sivan Velmathi^*

^*Corresponding author for this work

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Detection of cysteine is crucial as its deviation from normal concentration leads to various diseases. In this work, benzothiazole based chemodosimeter was synthesized to discriminate cysteine rapidly in the presence of other competing interferences. The probe exhibit ESIPT governed colorimetric and fluoremetric response on addition of cysteine via conjugate addition-cyclization reaction and it is confirmed by NMR and HRMS. The probe can detect lower concentration of cysteine with limit of detection 0.07 mu M in 2 min that assist the system to work well in biological systems. Hence the sensor was profitably employed in imaging of cysteine in HeLa cell line and variance in the redox state was also investigated.

Original language	English
Article number	112875
Number of pages	7
Journal	Journal of Photochemistry and Photobiology A: Chemistry
Volume	403
DOIs	https://doi.org/10.1016/j.jphotochem.2020.112875
State	Published - 1 Dec 2020

Keywords

ESIPT
Swift response
Biothiols
Discrimination
Redox imbalance
LARGE STOKES SHIFT
INTRAMOLECULAR PROTON-TRANSFER
HIGHLY SELECTIVE DETECTION
TURN-ON PROBE
FLUORESCENT-PROBE
ENDOPLASMIC-RETICULUM
HOMOCYSTEINE
STRESS
AGGREGATION
CHEMOSENSOR

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title = "ESIPT triggered swift determination of cysteine in HeLa cell line during redox imbalance",

abstract = "Detection of cysteine is crucial as its deviation from normal concentration leads to various diseases. In this work, benzothiazole based chemodosimeter was synthesized to discriminate cysteine rapidly in the presence of other competing interferences. The probe exhibit ESIPT governed colorimetric and fluoremetric response on addition of cysteine via conjugate addition-cyclization reaction and it is confirmed by NMR and HRMS. The probe can detect lower concentration of cysteine with limit of detection 0.07 mu M in 2 min that assist the system to work well in biological systems. Hence the sensor was profitably employed in imaging of cysteine in HeLa cell line and variance in the redox state was also investigated.",

keywords = "ESIPT, Swift response, Biothiols, Discrimination, Redox imbalance, LARGE STOKES SHIFT, INTRAMOLECULAR PROTON-TRANSFER, HIGHLY SELECTIVE DETECTION, TURN-ON PROBE, FLUORESCENT-PROBE, ENDOPLASMIC-RETICULUM, HOMOCYSTEINE, STRESS, AGGREGATION, CHEMOSENSOR",

author = "Kathiresan Anusuyadevi and Shu-Pao Wu and Sivan Velmathi",

year = "2020",

month = dec,

day = "1",

doi = "10.1016/j.jphotochem.2020.112875",

language = "English",

volume = "403",

journal = "Journal of Photochemistry and Photobiology A: Chemistry",

issn = "1010-6030",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - ESIPT triggered swift determination of cysteine in HeLa cell line during redox imbalance

AU - Anusuyadevi, Kathiresan

AU - Wu, Shu-Pao

AU - Velmathi, Sivan

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Detection of cysteine is crucial as its deviation from normal concentration leads to various diseases. In this work, benzothiazole based chemodosimeter was synthesized to discriminate cysteine rapidly in the presence of other competing interferences. The probe exhibit ESIPT governed colorimetric and fluoremetric response on addition of cysteine via conjugate addition-cyclization reaction and it is confirmed by NMR and HRMS. The probe can detect lower concentration of cysteine with limit of detection 0.07 mu M in 2 min that assist the system to work well in biological systems. Hence the sensor was profitably employed in imaging of cysteine in HeLa cell line and variance in the redox state was also investigated.

AB - Detection of cysteine is crucial as its deviation from normal concentration leads to various diseases. In this work, benzothiazole based chemodosimeter was synthesized to discriminate cysteine rapidly in the presence of other competing interferences. The probe exhibit ESIPT governed colorimetric and fluoremetric response on addition of cysteine via conjugate addition-cyclization reaction and it is confirmed by NMR and HRMS. The probe can detect lower concentration of cysteine with limit of detection 0.07 mu M in 2 min that assist the system to work well in biological systems. Hence the sensor was profitably employed in imaging of cysteine in HeLa cell line and variance in the redox state was also investigated.

KW - ESIPT

KW - Swift response

KW - Biothiols

KW - Discrimination

KW - Redox imbalance

KW - LARGE STOKES SHIFT

KW - INTRAMOLECULAR PROTON-TRANSFER

KW - HIGHLY SELECTIVE DETECTION

KW - TURN-ON PROBE

KW - FLUORESCENT-PROBE

KW - ENDOPLASMIC-RETICULUM

KW - HOMOCYSTEINE

KW - STRESS

KW - AGGREGATION

KW - CHEMOSENSOR

U2 - 10.1016/j.jphotochem.2020.112875

DO - 10.1016/j.jphotochem.2020.112875

M3 - Article

SN - 1010-6030

VL - 403

JO - Journal of Photochemistry and Photobiology A: Chemistry

JF - Journal of Photochemistry and Photobiology A: Chemistry

M1 - 112875

ER -

ESIPT triggered swift determination of cysteine in HeLa cell line during redox imbalance

Abstract

Keywords

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