ESIPT triggered swift determination of cysteine in HeLa cell line during redox imbalance

Kathiresan Anusuyadevi, Shu-Pao Wu, Sivan Velmathi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Detection of cysteine is crucial as its deviation from normal concentration leads to various diseases. In this work, benzothiazole based chemodosimeter was synthesized to discriminate cysteine rapidly in the presence of other competing interferences. The probe exhibit ESIPT governed colorimetric and fluoremetric response on addition of cysteine via conjugate addition-cyclization reaction and it is confirmed by NMR and HRMS. The probe can detect lower concentration of cysteine with limit of detection 0.07 mu M in 2 min that assist the system to work well in biological systems. Hence the sensor was profitably employed in imaging of cysteine in HeLa cell line and variance in the redox state was also investigated.

Original languageEnglish
Article number112875
Number of pages7
JournalJournal of Photochemistry and Photobiology A: Chemistry
Volume403
DOIs
StatePublished - 1 Dec 2020

Keywords

  • ESIPT
  • Swift response
  • Biothiols
  • Discrimination
  • Redox imbalance
  • LARGE STOKES SHIFT
  • INTRAMOLECULAR PROTON-TRANSFER
  • HIGHLY SELECTIVE DETECTION
  • TURN-ON PROBE
  • FLUORESCENT-PROBE
  • ENDOPLASMIC-RETICULUM
  • HOMOCYSTEINE
  • STRESS
  • AGGREGATION
  • CHEMOSENSOR

Fingerprint

Dive into the research topics of 'ESIPT triggered swift determination of cysteine in HeLa cell line during redox imbalance'. Together they form a unique fingerprint.

Cite this