Enhancement of gene therapy on hepatocellular carcinoma by sonoporation-parameter studies

Kun Che Tsai; Lih Hwa Hwang; Shu Jyuan Yang; Che Kang Liao; Win Li Lin; Ming Jium Shieh; Wen Shiang Chen

doi:10.1109/ULTSYM.2008.0135

Enhancement of gene therapy on hepatocellular carcinoma by sonoporation-parameter studies

Kun Che Tsai^*, Lih Hwa Hwang, Shu Jyuan Yang, Che Kang Liao, Win Li Lin, Ming Jium Shieh, Wen Shiang Chen

^*Corresponding author for this work

Institute of Microbiology and Immunology

Research output: Contribution to journal › Conference article › peer-review

Abstract

Ultrasound (US) is believed to be a novel and effective tool to locally deliver gene into target tumors. US can temporally change the permeability of cell membrane and thus enhance the delivery of naked DNA into cells. In this study, the gene transfection in tumor and muscle tissues was evaluated by changing the contrast agent concentrations and US exposure durations. The luciferase and IL-12 mixed with SonoVue® were injected intramuscularly (IM) or intratumorally (IT), followed by exposure to US of 20% in duty cycle and 2 W/cm² in intensity. The serum levels of IL-12 were determined by ELISA. Experimental results showed that both local and systemic expressions of the naked DNA in muscle are significantly higher than those in subcutaneous and liver tumors, and an order of muscle > subcutaneous tissue > liver tumor was found. Best transfection efficiency could be obtained by using 50% SonoVue in tumor and 30% in muscle after 10 min US exposure.

Original language	English
Article number	4803371
Pages (from-to)	562-565
Number of pages	4
Journal	Proceedings of the IEEE Ultrasonics Symposium
DOIs	https://doi.org/10.1109/ULTSYM.2008.0135
State	Published - 2008
Event	2008 IEEE International Ultrasonics Symposium, IUS 2008 - Beijing, China Duration: 2 Nov 2008 → 5 Nov 2008

Keywords

Gene transfection
Intramuscularly
Intratumorally
SonoVue®
Ultrasound

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10.1109/ULTSYM.2008.0135

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title = "Enhancement of gene therapy on hepatocellular carcinoma by sonoporation-parameter studies",

abstract = "Ultrasound (US) is believed to be a novel and effective tool to locally deliver gene into target tumors. US can temporally change the permeability of cell membrane and thus enhance the delivery of naked DNA into cells. In this study, the gene transfection in tumor and muscle tissues was evaluated by changing the contrast agent concentrations and US exposure durations. The luciferase and IL-12 mixed with SonoVue{\textregistered} were injected intramuscularly (IM) or intratumorally (IT), followed by exposure to US of 20% in duty cycle and 2 W/cm2 in intensity. The serum levels of IL-12 were determined by ELISA. Experimental results showed that both local and systemic expressions of the naked DNA in muscle are significantly higher than those in subcutaneous and liver tumors, and an order of muscle > subcutaneous tissue > liver tumor was found. Best transfection efficiency could be obtained by using 50% SonoVue in tumor and 30% in muscle after 10 min US exposure.",

keywords = "Gene transfection, Intramuscularly, Intratumorally, SonoVue{\textregistered}, Ultrasound",

author = "Tsai, {Kun Che} and Hwang, {Lih Hwa} and Yang, {Shu Jyuan} and Liao, {Che Kang} and Lin, {Win Li} and Shieh, {Ming Jium} and Chen, {Wen Shiang}",

year = "2008",

doi = "10.1109/ULTSYM.2008.0135",

language = "English",

pages = "562--565",

journal = "Proceedings of the IEEE Ultrasonics Symposium",

issn = "1051-0117",

publisher = "Institute of Electrical and Electronics Engineers Inc.",

note = "2008 IEEE International Ultrasonics Symposium, IUS 2008 ; Conference date: 02-11-2008 Through 05-11-2008",

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T1 - Enhancement of gene therapy on hepatocellular carcinoma by sonoporation-parameter studies

AU - Tsai, Kun Che

AU - Hwang, Lih Hwa

AU - Yang, Shu Jyuan

AU - Liao, Che Kang

AU - Lin, Win Li

AU - Shieh, Ming Jium

AU - Chen, Wen Shiang

PY - 2008

Y1 - 2008

N2 - Ultrasound (US) is believed to be a novel and effective tool to locally deliver gene into target tumors. US can temporally change the permeability of cell membrane and thus enhance the delivery of naked DNA into cells. In this study, the gene transfection in tumor and muscle tissues was evaluated by changing the contrast agent concentrations and US exposure durations. The luciferase and IL-12 mixed with SonoVue® were injected intramuscularly (IM) or intratumorally (IT), followed by exposure to US of 20% in duty cycle and 2 W/cm2 in intensity. The serum levels of IL-12 were determined by ELISA. Experimental results showed that both local and systemic expressions of the naked DNA in muscle are significantly higher than those in subcutaneous and liver tumors, and an order of muscle > subcutaneous tissue > liver tumor was found. Best transfection efficiency could be obtained by using 50% SonoVue in tumor and 30% in muscle after 10 min US exposure.

AB - Ultrasound (US) is believed to be a novel and effective tool to locally deliver gene into target tumors. US can temporally change the permeability of cell membrane and thus enhance the delivery of naked DNA into cells. In this study, the gene transfection in tumor and muscle tissues was evaluated by changing the contrast agent concentrations and US exposure durations. The luciferase and IL-12 mixed with SonoVue® were injected intramuscularly (IM) or intratumorally (IT), followed by exposure to US of 20% in duty cycle and 2 W/cm2 in intensity. The serum levels of IL-12 were determined by ELISA. Experimental results showed that both local and systemic expressions of the naked DNA in muscle are significantly higher than those in subcutaneous and liver tumors, and an order of muscle > subcutaneous tissue > liver tumor was found. Best transfection efficiency could be obtained by using 50% SonoVue in tumor and 30% in muscle after 10 min US exposure.

KW - Gene transfection

KW - Intramuscularly

KW - Intratumorally

KW - SonoVue®

KW - Ultrasound

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U2 - 10.1109/ULTSYM.2008.0135

DO - 10.1109/ULTSYM.2008.0135

M3 - Conference article

AN - SCOPUS:67649353273

SN - 1051-0117

SP - 562

EP - 565

JO - Proceedings of the IEEE Ultrasonics Symposium

JF - Proceedings of the IEEE Ultrasonics Symposium

M1 - 4803371

T2 - 2008 IEEE International Ultrasonics Symposium, IUS 2008

Y2 - 2 November 2008 through 5 November 2008

ER -

Enhancement of gene therapy on hepatocellular carcinoma by sonoporation-parameter studies

Abstract

Keywords

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