Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures

Keng Chi Cho; Chi Hsiang Lien; Chun Yu Lin; Chia Yuan Chang; Lynn L.H. Huang; Paul J. Campagnola; Chen Yuan Dong; Shean Jen Chen

doi:10.1364/OE.19.011732

Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures

Keng Chi Cho^*, Chi Hsiang Lien, Chun Yu Lin, Chia Yuan Chang, Lynn L.H. Huang, Paul J. Campagnola, Chen Yuan Dong, Shean Jen Chen

^*Corresponding author for this work

Institute of Imaging and Biomedical Photonics

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

In this study, the intensity of two-photon excited fluorescence (TPEF) of xanthene dye, Rose Bengal (RB), was significantly enhanced via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking (TPC) technique. The RB was utilized as the photoactivator in the TPC processing and the enhanced TPEF intensity correlates with the concentration of fabricated crosslinked BSA microstructures via the power control and pulse selection of the employed femtosecond laser. As a result, fabrication of three-dimensional BSA microstructures can be simultaneously monitored by the use of TPEF intensity. The crosslinked BSA microstructures synthesized may be used as an ordered biomaterial for fluorescence enhancement.

Original language	English
Pages (from-to)	11732-11739
Number of pages	8
Journal	Optics Express
Volume	19
Issue number	12
DOIs	https://doi.org/10.1364/OE.19.011732
State	Published - 6 Jun 2011

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title = "Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures",

abstract = "In this study, the intensity of two-photon excited fluorescence (TPEF) of xanthene dye, Rose Bengal (RB), was significantly enhanced via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking (TPC) technique. The RB was utilized as the photoactivator in the TPC processing and the enhanced TPEF intensity correlates with the concentration of fabricated crosslinked BSA microstructures via the power control and pulse selection of the employed femtosecond laser. As a result, fabrication of three-dimensional BSA microstructures can be simultaneously monitored by the use of TPEF intensity. The crosslinked BSA microstructures synthesized may be used as an ordered biomaterial for fluorescence enhancement.",

author = "Cho, {Keng Chi} and Lien, {Chi Hsiang} and Lin, {Chun Yu} and Chang, {Chia Yuan} and Huang, {Lynn L.H.} and Campagnola, {Paul J.} and Dong, {Chen Yuan} and Chen, {Shean Jen}",

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T1 - Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures

AU - Cho, Keng Chi

AU - Lien, Chi Hsiang

AU - Lin, Chun Yu

AU - Chang, Chia Yuan

AU - Huang, Lynn L.H.

AU - Campagnola, Paul J.

AU - Dong, Chen Yuan

AU - Chen, Shean Jen

PY - 2011/6/6

Y1 - 2011/6/6

N2 - In this study, the intensity of two-photon excited fluorescence (TPEF) of xanthene dye, Rose Bengal (RB), was significantly enhanced via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking (TPC) technique. The RB was utilized as the photoactivator in the TPC processing and the enhanced TPEF intensity correlates with the concentration of fabricated crosslinked BSA microstructures via the power control and pulse selection of the employed femtosecond laser. As a result, fabrication of three-dimensional BSA microstructures can be simultaneously monitored by the use of TPEF intensity. The crosslinked BSA microstructures synthesized may be used as an ordered biomaterial for fluorescence enhancement.

AB - In this study, the intensity of two-photon excited fluorescence (TPEF) of xanthene dye, Rose Bengal (RB), was significantly enhanced via bovine serum albumin (BSA) microstructures fabricated by the two-photon crosslinking (TPC) technique. The RB was utilized as the photoactivator in the TPC processing and the enhanced TPEF intensity correlates with the concentration of fabricated crosslinked BSA microstructures via the power control and pulse selection of the employed femtosecond laser. As a result, fabrication of three-dimensional BSA microstructures can be simultaneously monitored by the use of TPEF intensity. The crosslinked BSA microstructures synthesized may be used as an ordered biomaterial for fluorescence enhancement.

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DO - 10.1364/OE.19.011732

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AN - SCOPUS:79958183036

SN - 1094-4087

VL - 19

SP - 11732

EP - 11739

JO - Optics Express

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ER -

Enhanced two-photon excited fluorescence in three-dimensionally crosslinked bovine serum albumin microstructures

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