Enhanced Probe Bonding and Fluorescence Properties through Annealed Graphene Oxide Nanosheets

Chong You Chen, Hsien Yi Chiu, Shing Jyh Chang, Nai Lun Yeh, Chia Hao Chan, Chuan Chi Shih, Shiue Luen Chen, Jia Wei Yang, Chien Yu Huang, Guan Yu Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Graphene oxide (GO) has been widely used in biological sensing studies because of its excellent physical and chemical properties. In particular, the rich functional groups on the surface of GO can effectively enhance the bonding of biomolecules and serve as an efficient sensing substrate. However, when biomolecules are labeled with fluorescence, the GO interface affects the biomolecules by reducing the fluorescence properties and limiting their applications in biosensing. Here, we establish an annealed GO (aGO) substrate through the annealing process, which can effectively increase the bonding amount of a DNA probe because of the accumulation of oxygen atoms on the surface without significantly damaging the nanosheet structure. Furthermore, we prove that the aGO substrate can effectively maintain its fluorescence performance and stability by exposing more graphic domains. Overall, this study successfully verifies that GO's interface annealing modification can be used as an alternative innovative interface application in biosensing.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalACS Biomaterials Science and Engineering
StateAccepted/In press - 14 Feb 2022


  • DNA probe
  • fluorescence
  • graphene oxide
  • mild annealing
  • oxygen clustering


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