Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective

May Jywan Tsai; Yi Ming Arthur Chen; Ching Feng Weng; Dann Ying Liou; Hsin Chun Yang; Chien Hung Chen; Roanna I.Hsin Liao; Fu Shan Kuo; Chiuan Wen Chiu; Huai Sheng Kuo; Ming Chao Huang; Yi Lo Lin; Meng Jen Lee; Wen Chun Kuo; Wen Cheng Huang; Henrich Cheng

doi:10.1111/j.1749-6632.2009.05169.x

Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective

May Jywan Tsai, Yi Ming Arthur Chen, Ching Feng Weng, Dann Ying Liou, Hsin Chun Yang, Chien Hung Chen, Roanna I.Hsin Liao, Fu Shan Kuo, Chiuan Wen Chiu, Huai Sheng Kuo, Ming Chao Huang, Yi Lo Lin, Meng Jen Lee, Wen Chun Kuo, Wen Cheng Huang, Henrich Cheng^*

^*Corresponding author for this work

Institute of Pharmacology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

10 Scopus citations

Abstract

Glycine N-methyltransferase (GNMT) is the most abundant hepatic methyltransferase and plays important roles in regulating methyl group metabolism. In the central nervous system, GNMT expression is low and its function has not been revealed. The present study examines the effect of GNMT overexpression by adenovirus-mediated transfer in cortical mixed neuron-glial cultures. Infection of adenovirus encoding green fluorescence protein to cultures demonstrates high preference for non-neuronal cells. Optimal GNMT overexpression in cultures by adenoviral GNMT (Ad-GNMT) infection not only induces protein kinase C phosphorylation, but also increases neuronaloligodendroglial survival. Furthermore, these Ad-GNMT-infected cultures are significantly resistant to H₂O₂ toxicity and lipopolysaccharide stimulation. Conditioned media from Ad-GNMT-infected microglia also significantly enhance neuronal survival. Taken together, enhanced GNMT expression in mixed neuronal-glial cultures is neuroprotective, most likely mediated through non-neuronal cells.

Original language	English
Title of host publication	Neuroprotective Agents
Subtitle of host publication	Ninth International Conference
Publisher	Blackwell Publishing Inc.
Pages	194-203
Number of pages	10
ISBN (Print)	9781573317771
DOIs	https://doi.org/10.1111/j.1749-6632.2009.05169.x
State	Published - Jun 2010

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1199
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

Keywords

Adenovirus
GNMT
Lipopolysaccharide
Neuron-glia interaction

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10.1111/j.1749-6632.2009.05169.x

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Tsai, M. J., Chen, Y. M. A., Weng, C. F., Liou, D. Y., Yang, H. C., Chen, C. H., Liao, R. I. H., Kuo, F. S., Chiu, C. W., Kuo, H. S., Huang, M. C., Lin, Y. L., Lee, M. J., Kuo, W. C., Huang, W. C., & Cheng, H. (2010). Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective. In Neuroprotective Agents: Ninth International Conference (pp. 194-203). (Annals of the New York Academy of Sciences; Vol. 1199). Blackwell Publishing Inc.. https://doi.org/10.1111/j.1749-6632.2009.05169.x

@inproceedings{46ab285dd3984f4fbac9650067682f76,

title = "Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective",

abstract = "Glycine N-methyltransferase (GNMT) is the most abundant hepatic methyltransferase and plays important roles in regulating methyl group metabolism. In the central nervous system, GNMT expression is low and its function has not been revealed. The present study examines the effect of GNMT overexpression by adenovirus-mediated transfer in cortical mixed neuron-glial cultures. Infection of adenovirus encoding green fluorescence protein to cultures demonstrates high preference for non-neuronal cells. Optimal GNMT overexpression in cultures by adenoviral GNMT (Ad-GNMT) infection not only induces protein kinase C phosphorylation, but also increases neuronaloligodendroglial survival. Furthermore, these Ad-GNMT-infected cultures are significantly resistant to H2O2 toxicity and lipopolysaccharide stimulation. Conditioned media from Ad-GNMT-infected microglia also significantly enhance neuronal survival. Taken together, enhanced GNMT expression in mixed neuronal-glial cultures is neuroprotective, most likely mediated through non-neuronal cells.",

keywords = "Adenovirus, GNMT, Lipopolysaccharide, Neuron-glia interaction",

author = "Tsai, {May Jywan} and Chen, {Yi Ming Arthur} and Weng, {Ching Feng} and Liou, {Dann Ying} and Yang, {Hsin Chun} and Chen, {Chien Hung} and Liao, {Roanna I.Hsin} and Kuo, {Fu Shan} and Chiu, {Chiuan Wen} and Kuo, {Huai Sheng} and Huang, {Ming Chao} and Lin, {Yi Lo} and Lee, {Meng Jen} and Kuo, {Wen Chun} and Huang, {Wen Cheng} and Henrich Cheng",

year = "2010",

month = jun,

doi = "10.1111/j.1749-6632.2009.05169.x",

language = "English",

isbn = "9781573317771",

series = "Annals of the New York Academy of Sciences",

publisher = "Blackwell Publishing Inc.",

pages = "194--203",

booktitle = "Neuroprotective Agents",

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Tsai, MJ, Chen, YMA, Weng, CF, Liou, DY, Yang, HC, Chen, CH, Liao, RIH, Kuo, FS, Chiu, CW, Kuo, HS, Huang, MC, Lin, YL, Lee, MJ, Kuo, WC, Huang, WC & Cheng, H 2010, Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective. in Neuroprotective Agents: Ninth International Conference. Annals of the New York Academy of Sciences, vol. 1199, Blackwell Publishing Inc., pp. 194-203. https://doi.org/10.1111/j.1749-6632.2009.05169.x

Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective. / Tsai, May Jywan; Chen, Yi Ming Arthur; Weng, Ching Feng et al.
Neuroprotective Agents: Ninth International Conference. Blackwell Publishing Inc., 2010. p. 194-203 (Annals of the New York Academy of Sciences; Vol. 1199).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective

AU - Tsai, May Jywan

AU - Chen, Yi Ming Arthur

AU - Weng, Ching Feng

AU - Liou, Dann Ying

AU - Yang, Hsin Chun

AU - Chen, Chien Hung

AU - Liao, Roanna I.Hsin

AU - Kuo, Fu Shan

AU - Chiu, Chiuan Wen

AU - Kuo, Huai Sheng

AU - Huang, Ming Chao

AU - Lin, Yi Lo

AU - Lee, Meng Jen

AU - Kuo, Wen Chun

AU - Huang, Wen Cheng

AU - Cheng, Henrich

PY - 2010/6

Y1 - 2010/6

N2 - Glycine N-methyltransferase (GNMT) is the most abundant hepatic methyltransferase and plays important roles in regulating methyl group metabolism. In the central nervous system, GNMT expression is low and its function has not been revealed. The present study examines the effect of GNMT overexpression by adenovirus-mediated transfer in cortical mixed neuron-glial cultures. Infection of adenovirus encoding green fluorescence protein to cultures demonstrates high preference for non-neuronal cells. Optimal GNMT overexpression in cultures by adenoviral GNMT (Ad-GNMT) infection not only induces protein kinase C phosphorylation, but also increases neuronaloligodendroglial survival. Furthermore, these Ad-GNMT-infected cultures are significantly resistant to H2O2 toxicity and lipopolysaccharide stimulation. Conditioned media from Ad-GNMT-infected microglia also significantly enhance neuronal survival. Taken together, enhanced GNMT expression in mixed neuronal-glial cultures is neuroprotective, most likely mediated through non-neuronal cells.

AB - Glycine N-methyltransferase (GNMT) is the most abundant hepatic methyltransferase and plays important roles in regulating methyl group metabolism. In the central nervous system, GNMT expression is low and its function has not been revealed. The present study examines the effect of GNMT overexpression by adenovirus-mediated transfer in cortical mixed neuron-glial cultures. Infection of adenovirus encoding green fluorescence protein to cultures demonstrates high preference for non-neuronal cells. Optimal GNMT overexpression in cultures by adenoviral GNMT (Ad-GNMT) infection not only induces protein kinase C phosphorylation, but also increases neuronaloligodendroglial survival. Furthermore, these Ad-GNMT-infected cultures are significantly resistant to H2O2 toxicity and lipopolysaccharide stimulation. Conditioned media from Ad-GNMT-infected microglia also significantly enhance neuronal survival. Taken together, enhanced GNMT expression in mixed neuronal-glial cultures is neuroprotective, most likely mediated through non-neuronal cells.

KW - Adenovirus

KW - GNMT

KW - Lipopolysaccharide

KW - Neuron-glia interaction

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U2 - 10.1111/j.1749-6632.2009.05169.x

DO - 10.1111/j.1749-6632.2009.05169.x

M3 - Conference contribution

AN - SCOPUS:77954005840

SN - 9781573317771

T3 - Annals of the New York Academy of Sciences

SP - 194

EP - 203

BT - Neuroprotective Agents

PB - Blackwell Publishing Inc.

ER -

Tsai MJ, Chen YMA, Weng CF, Liou DY, Yang HC, Chen CH et al. Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective. In Neuroprotective Agents: Ninth International Conference. Blackwell Publishing Inc. 2010. p. 194-203. (Annals of the New York Academy of Sciences). doi: 10.1111/j.1749-6632.2009.05169.x

Enhanced expression of glycine N-methyltransferase by adenovirus-mediated gene transfer in CNS culture is neuroprotective

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