Enhanced bioconjugation on sputtered palladium nano-thin-film electrode

Chia Yu Chang, Wei Chen, Chien Hao Su, Pang Chia Chang, Yun Tzu Huang, Kuo Chen Hsu, Chiun-Jye Yuan, Chia-Ching Chang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


A highly reactive surface with an enhanced ability for chemical bonding relies on the presence of specifically coordinated atoms and step edges at the surface. In this study, an electrode with a unique Stranski-Krastanov-like thin film, with an epitaxial sputtering of a palladium (Pd) nanoparticle double layer on the polyethylene terephthalate substrate, was developed. On the surface of this flexible Pd-nano-thin-film (NTF) electrode with a (1 1 1) containing surface, DNA probes can be quickly immobilized in as short a period as 20 min, which is 24 times faster than that on the gold electrode. A DNA-based anticancer compound (ACC) sensing and screening process that would use the DNA functionalized Pd-NTF electrode as the biosensor was then proposed. Interestingly, the developed biosensor could detect DNA and ACCs, such as doxorubicin, tetra-O-methyl nordihydroguaiaretic acid, and Taxol via interactions with solutions containing 1 μl ACCs within 11 min, and the sensitivity of the ACC solution is ∼0.1 μM (∼36 pg per-test), as detected by electrochemical impedance spectroscopy. Moreover, this highly reactive surface can be used in regular sensors and other interfaces, in scientific applications.

Original languageEnglish
Article number093702
Number of pages5
JournalApplied Physics Letters
Issue number9
StatePublished - 4 Mar 2019


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