TY - JOUR
T1 - Enhanced bioconjugation on sputtered palladium nano-thin-film electrode
AU - Chang, Chia Yu
AU - Chen, Wei
AU - Su, Chien Hao
AU - Chang, Pang Chia
AU - Huang, Yun Tzu
AU - Hsu, Kuo Chen
AU - Yuan, Chiun-Jye
AU - Chang, Chia-Ching
PY - 2019/3/4
Y1 - 2019/3/4
N2 - A highly reactive surface with an enhanced ability for chemical bonding relies on the presence of specifically coordinated atoms and step edges at the surface. In this study, an electrode with a unique Stranski-Krastanov-like thin film, with an epitaxial sputtering of a palladium (Pd) nanoparticle double layer on the polyethylene terephthalate substrate, was developed. On the surface of this flexible Pd-nano-thin-film (NTF) electrode with a (1 1 1) containing surface, DNA probes can be quickly immobilized in as short a period as 20 min, which is 24 times faster than that on the gold electrode. A DNA-based anticancer compound (ACC) sensing and screening process that would use the DNA functionalized Pd-NTF electrode as the biosensor was then proposed. Interestingly, the developed biosensor could detect DNA and ACCs, such as doxorubicin, tetra-O-methyl nordihydroguaiaretic acid, and Taxol via interactions with solutions containing 1 μl ACCs within 11 min, and the sensitivity of the ACC solution is ∼0.1 μM (∼36 pg per-test), as detected by electrochemical impedance spectroscopy. Moreover, this highly reactive surface can be used in regular sensors and other interfaces, in scientific applications.
AB - A highly reactive surface with an enhanced ability for chemical bonding relies on the presence of specifically coordinated atoms and step edges at the surface. In this study, an electrode with a unique Stranski-Krastanov-like thin film, with an epitaxial sputtering of a palladium (Pd) nanoparticle double layer on the polyethylene terephthalate substrate, was developed. On the surface of this flexible Pd-nano-thin-film (NTF) electrode with a (1 1 1) containing surface, DNA probes can be quickly immobilized in as short a period as 20 min, which is 24 times faster than that on the gold electrode. A DNA-based anticancer compound (ACC) sensing and screening process that would use the DNA functionalized Pd-NTF electrode as the biosensor was then proposed. Interestingly, the developed biosensor could detect DNA and ACCs, such as doxorubicin, tetra-O-methyl nordihydroguaiaretic acid, and Taxol via interactions with solutions containing 1 μl ACCs within 11 min, and the sensitivity of the ACC solution is ∼0.1 μM (∼36 pg per-test), as detected by electrochemical impedance spectroscopy. Moreover, this highly reactive surface can be used in regular sensors and other interfaces, in scientific applications.
UR - http://www.scopus.com/inward/record.url?scp=85062734573&partnerID=8YFLogxK
U2 - 10.1063/1.5087030
DO - 10.1063/1.5087030
M3 - Article
AN - SCOPUS:85062734573
SN - 0003-6951
VL - 114
JO - Applied Physics Letters
JF - Applied Physics Letters
IS - 9
M1 - 093702
ER -