Emergence of Qnr determinants in human Salmonella isolates in Taiwan

Jiunn Jong Wu; Wen Chien Ko; Chien Shun Chiou; Hung Mo Chen; Li Ron Wang; Jing Jou Yan

doi:10.1093/jac/dkn426

Emergence of Qnr determinants in human Salmonella isolates in Taiwan

Jiunn Jong Wu
, Wen Chien Ko
, Chien Shun Chiou
, Hung Mo Chen
, Li Ron Wang
, Jing Jou Yan^*

^*Corresponding author for this work

Department of Biotechnology and Laboratory Science in Medicine

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Objectives: The aim of this study was to determine the prevalence and characteristics of qnr-carrying Salmonella isolates from humans in southern Taiwan. Methods: A total of 446 Salmonella isolates collected between 2003 and 2006 were screened for qnrA, qnrB and qnrS by PCR experiments. Genetic structures of qnr were determined by PCR-based methods or direct sequencing of plasmid DNA. Results: qnrB2 and qnrS1 were detected in two serovar Enteritidis isolates and two serovar Typhimurium isolates, respectively. One qnrS1-positive isolate was found to produce the CMY-2 AmpC enzyme. qnrS1 was identified on a 10 kb plasmid, which exhibited >99% nucleotide sequence identity with plasmid TPqnrS-1a reported from the UK. qnrB2 was found in a complex sul1-type class 1 integron on a >100 kb plasmid. Conclusions: This study demonstrated the occurrence of qnrB2 and qnrS1 in Salmonella for the first time in Taiwan and characterized their genetic structures.

Original language	English
Pages (from-to)	1269-1272
Number of pages	4
Journal	Journal of Antimicrobial Chemotherapy
Volume	62
Issue number	6
DOIs	https://doi.org/10.1093/jac/dkn426
State	Published - 2008

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cephalosporinases
Plasmid-mediated quinolone resistance
Quinolones

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@article{7f157884500748bc822223479aecd736,

title = "Emergence of Qnr determinants in human Salmonella isolates in Taiwan",

abstract = "Objectives: The aim of this study was to determine the prevalence and characteristics of qnr-carrying Salmonella isolates from humans in southern Taiwan. Methods: A total of 446 Salmonella isolates collected between 2003 and 2006 were screened for qnrA, qnrB and qnrS by PCR experiments. Genetic structures of qnr were determined by PCR-based methods or direct sequencing of plasmid DNA. Results: qnrB2 and qnrS1 were detected in two serovar Enteritidis isolates and two serovar Typhimurium isolates, respectively. One qnrS1-positive isolate was found to produce the CMY-2 AmpC enzyme. qnrS1 was identified on a 10 kb plasmid, which exhibited >99\% nucleotide sequence identity with plasmid TPqnrS-1a reported from the UK. qnrB2 was found in a complex sul1-type class 1 integron on a >100 kb plasmid. Conclusions: This study demonstrated the occurrence of qnrB2 and qnrS1 in Salmonella for the first time in Taiwan and characterized their genetic structures.",

keywords = "Cephalosporinases, Plasmid-mediated quinolone resistance, Quinolones",

author = "Wu, \{Jiunn Jong\} and Ko, \{Wen Chien\} and Chiou, \{Chien Shun\} and Chen, \{Hung Mo\} and Wang, \{Li Ron\} and Yan, \{Jing Jou\}",

note = "Funding Information: This work was funded by grants NSC 96-2320-B-006-054 from the National Science Council, Taiwan and NCKUH 9703004 from the National Cheng Kung University Hospital, Taiwan.",

year = "2008",

doi = "10.1093/jac/dkn426",

language = "English",

volume = "62",

pages = "1269--1272",

journal = "Journal of Antimicrobial Chemotherapy",

issn = "0305-7453",

publisher = "Oxford University Press",

number = "6",

}

TY - JOUR

T1 - Emergence of Qnr determinants in human Salmonella isolates in Taiwan

AU - Wu, Jiunn Jong

AU - Ko, Wen Chien

AU - Chiou, Chien Shun

AU - Chen, Hung Mo

AU - Wang, Li Ron

AU - Yan, Jing Jou

N1 - Funding Information: This work was funded by grants NSC 96-2320-B-006-054 from the National Science Council, Taiwan and NCKUH 9703004 from the National Cheng Kung University Hospital, Taiwan.

PY - 2008

Y1 - 2008

N2 - Objectives: The aim of this study was to determine the prevalence and characteristics of qnr-carrying Salmonella isolates from humans in southern Taiwan. Methods: A total of 446 Salmonella isolates collected between 2003 and 2006 were screened for qnrA, qnrB and qnrS by PCR experiments. Genetic structures of qnr were determined by PCR-based methods or direct sequencing of plasmid DNA. Results: qnrB2 and qnrS1 were detected in two serovar Enteritidis isolates and two serovar Typhimurium isolates, respectively. One qnrS1-positive isolate was found to produce the CMY-2 AmpC enzyme. qnrS1 was identified on a 10 kb plasmid, which exhibited >99% nucleotide sequence identity with plasmid TPqnrS-1a reported from the UK. qnrB2 was found in a complex sul1-type class 1 integron on a >100 kb plasmid. Conclusions: This study demonstrated the occurrence of qnrB2 and qnrS1 in Salmonella for the first time in Taiwan and characterized their genetic structures.

AB - Objectives: The aim of this study was to determine the prevalence and characteristics of qnr-carrying Salmonella isolates from humans in southern Taiwan. Methods: A total of 446 Salmonella isolates collected between 2003 and 2006 were screened for qnrA, qnrB and qnrS by PCR experiments. Genetic structures of qnr were determined by PCR-based methods or direct sequencing of plasmid DNA. Results: qnrB2 and qnrS1 were detected in two serovar Enteritidis isolates and two serovar Typhimurium isolates, respectively. One qnrS1-positive isolate was found to produce the CMY-2 AmpC enzyme. qnrS1 was identified on a 10 kb plasmid, which exhibited >99% nucleotide sequence identity with plasmid TPqnrS-1a reported from the UK. qnrB2 was found in a complex sul1-type class 1 integron on a >100 kb plasmid. Conclusions: This study demonstrated the occurrence of qnrB2 and qnrS1 in Salmonella for the first time in Taiwan and characterized their genetic structures.

KW - Cephalosporinases

KW - Plasmid-mediated quinolone resistance

KW - Quinolones

UR - https://www.scopus.com/pages/publications/56649086858

U2 - 10.1093/jac/dkn426

DO - 10.1093/jac/dkn426

M3 - Article

C2 - 18957397

AN - SCOPUS:56649086858

SN - 0305-7453

VL - 62

SP - 1269

EP - 1272

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 6

ER -

Emergence of Qnr determinants in human Salmonella isolates in Taiwan

Abstract

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Keywords

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