Electrochemical biosensor based on an atomic layered composite of graphene oxide/graphene as an electrode material towards selective and sensitive detection of miRNA-21

Min Shin Huang, Mani Govindasamy, Sathishkumar Chinnapaiyan, Yi Ting Lin, Shao Yang Lu, Seiji Samukawa, Chi Hsien Huang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Development of an efficient technique towards the detection of miRNAs is highly significant for the timely diagnosis and prognosis of cancers. In this study, we utilized the chemical vapor deposition (CVD) technique to grow bilayer graphene (BLG) on copper foil and subsequently transferred the BLG to fluorine doped tin oxide (FTO) substrate via a facile wet transfer technique. The upper layer of the BLG was modified to graphene oxide (GO) with a low damage plasma treatment (LDPT) process to serve as a biomolecule receiving layer and the lower graphene layer (G) serves as an electron transport layer. The developed GO/G/FTO platform offers superior sensing ability towards the miRNA-21. The results demonstrated that the proposed GO/G/FTO substrate detect the miRNA-21 with a wide linear range (10 fM ∼ 1 nM), low limit of detection (3.18 fM), high sensitivity (0.6 mApM−1cm−2), selectivity and stability via the differential pulse voltammetry method. Additionally, the sensors can efficiently discriminate the target miRNA-21 from its complementary base mismatched counterparts. The proposed approach serves as an excellent tool for the early diagnosis of diseases.

Original languageEnglish
Article number110112
JournalMicrochemical Journal
Volume199
DOIs
StatePublished - Apr 2024

Keywords

  • Atomic layer oxidation
  • Biosensor
  • Cancer biomarkers
  • Electrochemical
  • Graphene oxide
  • Plasma treatment

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