Electrical stimulation to promote osteogenesis using conductive polypyrrole films

Wei Wen Hu*, Yi Ting Hsu, Yu Che Cheng, Chuan Li, Ruoh Chyu Ruaan, Chih Cheng Chien, Chih Ang Chung, Chia Wen Tsao

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

111 Scopus citations


In this study, we developed an electrical cell culture and monitoring device. Polypyrrole (PPy) films with different resistances were fabricated as conductive surfaces to investigate the effect of substrate-mediated electrical stimulation. The physical and chemical properties of the devices, as well as their biocompatibilities, were thoroughly evaluated. These PPy films had a dark but transparent appearance, on which the surface cells could be easily observed. After treating with the osteogenic medium, rat bone marrow stromal cells cultured on the PPy films differentiated into osteoblasts. The cells grown on the PPy films had up-regulated osteogenic markers, and an alkaline phosphatase activity assay showed that the PPy films accelerated cell differentiation. Alizarin red staining and calcium analysis suggested that the PPy films promoted osteogenesis. Finally, PPy films were subjected to a constant electric field to elucidate the effect of electrical stimulation on osteogenesis. Compared with the untreated group, electrical stimulation improved calcium deposition in the extracellular matrix. Furthermore, PPy films with lower resistances allowed larger currents to stimulate the surface cells, which resulted in higher levels of mineralization. Overall, these results indicated that this system exhibited superior electroactivity with controllable electrical resistance and that it can be coated directly to produce medical devices with a transparent appearance, which should be beneficial for research on electrical stimulation for tissue regeneration.

Original languageEnglish
Pages (from-to)28-36
Number of pages9
JournalMaterials Science and Engineering C
Issue number1
StatePublished - 1 Apr 2014


  • Bone marrow stromal cells
  • Direct current field
  • Electrical stimulation
  • Mineralization
  • Osteogenesis
  • Polypyrrole


Dive into the research topics of 'Electrical stimulation to promote osteogenesis using conductive polypyrrole films'. Together they form a unique fingerprint.

Cite this