Effects of ethanol and nomifensine on NE clearance in the cerebellum of young and aged Fischer 344 rats

Anya M.Y. Lin; Paula C. Bickford; Michael R. Palmer; Elizabeth J. Cline; Greg A. Gerhardt

doi:10.1016/S0006-8993(97)00229-1

Effects of ethanol and nomifensine on NE clearance in the cerebellum of young and aged Fischer 344 rats

Anya M.Y. Lin, Paula C. Bickford, Michael R. Palmer, Elizabeth J. Cline, Greg A. Gerhardt^*

^*Corresponding author for this work

Faculty of Pharmacy

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Rapid chronoamperometric recordings coupled with local application of drugs by pressure ejection were used to investigate the effects of nomifensine and ethanol (EtOH) on exogenous norepinephrine (NE) clearance in the cerebellum of young (5-month-old) and aged (24-26-month-old) male Fischer 344 rats. In the young rats, local nomifensine application prolonged exogenous NE clearance, indicating transporter mediated uptake inhibition. NE clearance was modestly but significantly prolonged in the aged rats as compared to the young rats, suggesting less efficient uptake. Consistent with this, there was little effect of nomifensine on NE clearance in the aged rats. In contrast to the effect of nomifensine, EtOH inhibited NE clearance in both young and aged rats. These data further support the hypothesis that one effect of EtOH in cerebellar NE systems is inhibition of NE uptake into NE-containing nerve terminals, and they also demonstrate that the effect of nomifensine on exogenous NE clearance in vivo in the cerebellum is altered by the aging process, while the effect of EtOH is not.

Original language	English
Pages (from-to)	287-292
Number of pages	6
Journal	Brain Research
Volume	756
Issue number	1-2
DOIs	https://doi.org/10.1016/S0006-8993(97)00229-1
State	Published - 9 May 1997

Keywords

Age
Cerebellar cortex
Ethanol
Fischer 344 rat
Nomifensine
Norepinephrine
Uptake

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10.1016/S0006-8993(97)00229-1

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title = "Effects of ethanol and nomifensine on NE clearance in the cerebellum of young and aged Fischer 344 rats",

abstract = "Rapid chronoamperometric recordings coupled with local application of drugs by pressure ejection were used to investigate the effects of nomifensine and ethanol (EtOH) on exogenous norepinephrine (NE) clearance in the cerebellum of young (5-month-old) and aged (24-26-month-old) male Fischer 344 rats. In the young rats, local nomifensine application prolonged exogenous NE clearance, indicating transporter mediated uptake inhibition. NE clearance was modestly but significantly prolonged in the aged rats as compared to the young rats, suggesting less efficient uptake. Consistent with this, there was little effect of nomifensine on NE clearance in the aged rats. In contrast to the effect of nomifensine, EtOH inhibited NE clearance in both young and aged rats. These data further support the hypothesis that one effect of EtOH in cerebellar NE systems is inhibition of NE uptake into NE-containing nerve terminals, and they also demonstrate that the effect of nomifensine on exogenous NE clearance in vivo in the cerebellum is altered by the aging process, while the effect of EtOH is not.",

keywords = "Age, Cerebellar cortex, Ethanol, Fischer 344 rat, Nomifensine, Norepinephrine, Uptake",

author = "Lin, {Anya M.Y.} and Bickford, {Paula C.} and Palmer, {Michael R.} and Cline, {Elizabeth J.} and Gerhardt, {Greg A.}",

note = "Funding Information: The authors thank David Young for his help with statistical analysis. This work was supported by USPHS grants AA05915, AA00102 and AG06434 and the Veteran Administration Medical Research Services. M.R.P is supported by an ADAMHA Research Scientist Development Award. A.M.-Y. Lin is supported by an Educational grant #27051F from National Science Council and Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, The Republic of China. G.A.G. also received support from a Level II Research Scientist Development Award, MH01245. ",

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T1 - Effects of ethanol and nomifensine on NE clearance in the cerebellum of young and aged Fischer 344 rats

AU - Lin, Anya M.Y.

AU - Bickford, Paula C.

AU - Palmer, Michael R.

AU - Cline, Elizabeth J.

AU - Gerhardt, Greg A.

N1 - Funding Information: The authors thank David Young for his help with statistical analysis. This work was supported by USPHS grants AA05915, AA00102 and AG06434 and the Veteran Administration Medical Research Services. M.R.P is supported by an ADAMHA Research Scientist Development Award. A.M.-Y. Lin is supported by an Educational grant #27051F from National Science Council and Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan, The Republic of China. G.A.G. also received support from a Level II Research Scientist Development Award, MH01245.

PY - 1997/5/9

Y1 - 1997/5/9

N2 - Rapid chronoamperometric recordings coupled with local application of drugs by pressure ejection were used to investigate the effects of nomifensine and ethanol (EtOH) on exogenous norepinephrine (NE) clearance in the cerebellum of young (5-month-old) and aged (24-26-month-old) male Fischer 344 rats. In the young rats, local nomifensine application prolonged exogenous NE clearance, indicating transporter mediated uptake inhibition. NE clearance was modestly but significantly prolonged in the aged rats as compared to the young rats, suggesting less efficient uptake. Consistent with this, there was little effect of nomifensine on NE clearance in the aged rats. In contrast to the effect of nomifensine, EtOH inhibited NE clearance in both young and aged rats. These data further support the hypothesis that one effect of EtOH in cerebellar NE systems is inhibition of NE uptake into NE-containing nerve terminals, and they also demonstrate that the effect of nomifensine on exogenous NE clearance in vivo in the cerebellum is altered by the aging process, while the effect of EtOH is not.

AB - Rapid chronoamperometric recordings coupled with local application of drugs by pressure ejection were used to investigate the effects of nomifensine and ethanol (EtOH) on exogenous norepinephrine (NE) clearance in the cerebellum of young (5-month-old) and aged (24-26-month-old) male Fischer 344 rats. In the young rats, local nomifensine application prolonged exogenous NE clearance, indicating transporter mediated uptake inhibition. NE clearance was modestly but significantly prolonged in the aged rats as compared to the young rats, suggesting less efficient uptake. Consistent with this, there was little effect of nomifensine on NE clearance in the aged rats. In contrast to the effect of nomifensine, EtOH inhibited NE clearance in both young and aged rats. These data further support the hypothesis that one effect of EtOH in cerebellar NE systems is inhibition of NE uptake into NE-containing nerve terminals, and they also demonstrate that the effect of nomifensine on exogenous NE clearance in vivo in the cerebellum is altered by the aging process, while the effect of EtOH is not.

KW - Age

KW - Cerebellar cortex

KW - Ethanol

KW - Fischer 344 rat

KW - Nomifensine

KW - Norepinephrine

KW - Uptake

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JO - Brain Research

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ER -

Effects of ethanol and nomifensine on NE clearance in the cerebellum of young and aged Fischer 344 rats

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