Effects of anti-triadin antibody on Ca2+ release from sarcoplasmic reticulum

Neil R. Brandt, Anthony H. Caswell, J. P. Brunschwig, Jaw Jou Kang, Bozena Antoniu, Noriaki Ikemoto*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

The monoclonal antibody, mAb GE 4.90, raised against triadin, a 95 kDa protein of sarcoplasmic reticulum (SR), inhibits the slow phase of Ca2+ release from SR following depolarization of the T-tubule moiety of the triad. The antibody has virtually no effect on the fast phase of depolarization-induced Ca2+ release nor on caffeine-induced Ca2+ release. Since the slow phase of depolarization-induced Ca2+ release is also inhibited by dihydropyridines (DHP), these results suggest that triadin may be involved in the functional coupling between the DHP receptor and the SR Ca2+ channel.

Original languageEnglish
Pages (from-to)57-59
Number of pages3
JournalFEBS Letters
Volume299
Issue number1
DOIs
StatePublished - 2 Mar 1992

Keywords

  • Ca release
  • Excitation-contraction coupling
  • Sarcoplasmic reticulum
  • Triadin

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