Effect of C-type lectin 16 on dengue virus infection in Aedes aegypti salivary glands

Ya Chen Chang; Wei Liang Liu; Pai Hsiang Fang; Jian Chiuan Li; Kun Lin Liu; Jau Ling Huang; Hsin Wei Chen; Chun Hong Chen; Chih Fei Kao

doi:10.1093/pnasnexus/pgae188

Effect of C-type lectin 16 on dengue virus infection in Aedes aegypti salivary glands

Ya Chen Chang, Wei Liang Liu, Pai Hsiang Fang, Jian Chiuan Li, Kun Lin Liu, Jau Ling Huang, Hsin Wei Chen, Chun Hong Chen^*, Chih Fei Kao

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

C-type lectins (CTLs) are a family of carbohydrate-binding proteins and an important component of mosquito saliva. Although CTLs play key roles in immune activation and viral pathogenesis, little is known about their role in regulating dengue virus (DENV) infection and transmission. In this study, we established a homozygous CTL16 knockout Aedes aegypti mutant line using CRISPR/Cas9 to study the interaction between CTL16 and viruses in mosquito vectors. Furthermore, mouse experiments were conducted to confirm the transmission of DENV by CTL16^−/− A. aegypti mutants. We found that CTL16 was mainly expressed in the medial lobe of the salivary glands (SGs) in female A. aegypti. CTL16 knockout increased DENV replication and accumulation in the SGs of female A. aegypti, suggesting that CTL16 plays an important role in DENV transmission. We also found a reduced expression of immunodeficiency and Janus kinase/signal transducer and activator of transcription pathway components correlated with increased DENV viral titer, infection rate, and transmission efficiency in the CTL16 mutant strain. The findings of this study provide insights not only for guiding future investigations on the influence of CTLs on immune responses in mosquitoes but also for developing novel mutants that can be used as vector control tools.

Original language	English
Article number	pgae188
Journal	PNAS Nexus
Volume	3
Issue number	5
DOIs	https://doi.org/10.1093/pnasnexus/pgae188
State	Published - 1 May 2024

Keywords

Aedes aegypti
C-type lectins
CRISPR/Cas9
CTL16
transmission

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/pnasnexus/pgae188

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title = "Effect of C-type lectin 16 on dengue virus infection in Aedes aegypti salivary glands",

abstract = "C-type lectins (CTLs) are a family of carbohydrate-binding proteins and an important component of mosquito saliva. Although CTLs play key roles in immune activation and viral pathogenesis, little is known about their role in regulating dengue virus (DENV) infection and transmission. In this study, we established a homozygous CTL16 knockout Aedes aegypti mutant line using CRISPR/Cas9 to study the interaction between CTL16 and viruses in mosquito vectors. Furthermore, mouse experiments were conducted to confirm the transmission of DENV by CTL16−/− A. aegypti mutants. We found that CTL16 was mainly expressed in the medial lobe of the salivary glands (SGs) in female A. aegypti. CTL16 knockout increased DENV replication and accumulation in the SGs of female A. aegypti, suggesting that CTL16 plays an important role in DENV transmission. We also found a reduced expression of immunodeficiency and Janus kinase/signal transducer and activator of transcription pathway components correlated with increased DENV viral titer, infection rate, and transmission efficiency in the CTL16 mutant strain. The findings of this study provide insights not only for guiding future investigations on the influence of CTLs on immune responses in mosquitoes but also for developing novel mutants that can be used as vector control tools.",

keywords = "Aedes aegypti, C-type lectins, CRISPR/Cas9, CTL16, transmission",

author = "Chang, {Ya Chen} and Liu, {Wei Liang} and Fang, {Pai Hsiang} and Li, {Jian Chiuan} and Liu, {Kun Lin} and Huang, {Jau Ling} and Chen, {Hsin Wei} and Chen, {Chun Hong} and Kao, {Chih Fei}",

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AU - Chang, Ya Chen

AU - Liu, Wei Liang

AU - Fang, Pai Hsiang

AU - Li, Jian Chiuan

AU - Liu, Kun Lin

AU - Huang, Jau Ling

AU - Chen, Hsin Wei

AU - Chen, Chun Hong

AU - Kao, Chih Fei

N1 - Publisher Copyright: © The Author(s) 2024.

PY - 2024/5/1

Y1 - 2024/5/1

N2 - C-type lectins (CTLs) are a family of carbohydrate-binding proteins and an important component of mosquito saliva. Although CTLs play key roles in immune activation and viral pathogenesis, little is known about their role in regulating dengue virus (DENV) infection and transmission. In this study, we established a homozygous CTL16 knockout Aedes aegypti mutant line using CRISPR/Cas9 to study the interaction between CTL16 and viruses in mosquito vectors. Furthermore, mouse experiments were conducted to confirm the transmission of DENV by CTL16−/− A. aegypti mutants. We found that CTL16 was mainly expressed in the medial lobe of the salivary glands (SGs) in female A. aegypti. CTL16 knockout increased DENV replication and accumulation in the SGs of female A. aegypti, suggesting that CTL16 plays an important role in DENV transmission. We also found a reduced expression of immunodeficiency and Janus kinase/signal transducer and activator of transcription pathway components correlated with increased DENV viral titer, infection rate, and transmission efficiency in the CTL16 mutant strain. The findings of this study provide insights not only for guiding future investigations on the influence of CTLs on immune responses in mosquitoes but also for developing novel mutants that can be used as vector control tools.

AB - C-type lectins (CTLs) are a family of carbohydrate-binding proteins and an important component of mosquito saliva. Although CTLs play key roles in immune activation and viral pathogenesis, little is known about their role in regulating dengue virus (DENV) infection and transmission. In this study, we established a homozygous CTL16 knockout Aedes aegypti mutant line using CRISPR/Cas9 to study the interaction between CTL16 and viruses in mosquito vectors. Furthermore, mouse experiments were conducted to confirm the transmission of DENV by CTL16−/− A. aegypti mutants. We found that CTL16 was mainly expressed in the medial lobe of the salivary glands (SGs) in female A. aegypti. CTL16 knockout increased DENV replication and accumulation in the SGs of female A. aegypti, suggesting that CTL16 plays an important role in DENV transmission. We also found a reduced expression of immunodeficiency and Janus kinase/signal transducer and activator of transcription pathway components correlated with increased DENV viral titer, infection rate, and transmission efficiency in the CTL16 mutant strain. The findings of this study provide insights not only for guiding future investigations on the influence of CTLs on immune responses in mosquitoes but also for developing novel mutants that can be used as vector control tools.

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Effect of C-type lectin 16 on dengue virus infection in Aedes aegypti salivary glands

Abstract

Keywords

UN SDGs

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