Dual-Responsive Benzo-Hemicyanine-Based Fluorescent Probe for Detection of Cyanide and Hydrogen Sulfide: Real-Time Application in Identification of Food Spoilage

Kuppan Magesh, Natarajan Vijay, Shu Pao Wu, Sivan Velmathi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Colorimetric and fluorescent probes have received a lot of attention for detecting lethal analytes in realistic systems and in living things. Herein, a dual-approachable Benzo-hemicyaninebased red-emitting fluorescent probe PBiSMe, for distinct and instantaneous detection of CN- and HS- was synthesized. The PBiSMe emitted red fluorescence (570 nm) can switch to turn-off (570 nm) and blue fluorescence (465 nm) in response to CN- and HS-, respectively. Other nucleophilic reagents, such as reactive sulfur species (RSS) and anions, have no contact or interference with the probe; instead, a unique approach is undertaken to exclusively interact with CN- and HS- over a wide pH range. The measured detection limits for CN- (0.43 μM) and HS- (0.22 μM) ions are lower than the World Health Organization’s (WHO) recommended levels in drinking water. We confirmed 1:1 stoichiometry ratio using Job’s plot and observed good quantum yield for both analytes. The probe-coated paper strips were used to detect the H2S gas produced by food spoilage (such as eggs, raw meat, and fish) via an eye-catching visual response. Moreover, fluorescence bioimaging studies of living cells was done to confirm the probe’s potential by monitoring the presence of CN- and HS- in a living system.

Original languageEnglish
Pages (from-to)1190-1200
Number of pages11
JournalJournal of Agricultural and Food Chemistry
Volume71
Issue number2
DOIs
StatePublished - 18 Jan 2023

Keywords

  • cyanide
  • food spoilage
  • hydrogen sulfide
  • nucleophilic addition
  • paper strips

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