@inbook{cd2777349a2543f990cde78488aa3a94,
title = "Directed Differentiation of Human iPSCs into Microglia-Like Cells Using Defined Transcription Factors",
abstract = "The generation of a homogeneous population of microglia from human induced pluripotent stem cells (hiPSCs) is crucial to modeling neurological disorders, as well as the carrying out of drug screening and toxicity testing. Here, we provide a stepwise protocol for the simple, robust, and efficient differentiation of hiPSCs into microglia-like cells (iMGs) by overexpression of SPI1 and CEBPA. This protocol details hiPSC culture, lentivirus production, lentivirus delivery, and, finally, the differentiation and validation of the iMG cells.",
keywords = "CEBPA, Induced pluripotent stem cells, Microglia, SPI1, Transcription factor-based differentiation",
author = "Chen, {Shih Wei} and Wong, {Yu Hui}",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-3287-1_5",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "53--68",
booktitle = "Methods in Molecular Biology",
}