TY - JOUR
T1 - Direct observation of single molecule conformational change of tight-turn paperclip DNA triplex in solution
AU - Liu, Ching Ping
AU - Wey, Ming Tsai
AU - Chang, Chia-Ching
AU - Kan, Lou Sing
PY - 2009/10
Y1 - 2009/10
N2 - DNA triplex modulates gene expression by forming stable conformation in physiological condition. However, it is not feasible to observe this unique molecular structure of large molecule with 54 oligodeoxynucleotides directly by conventional nuclear magnetic approach. In this study, we observed directly single molecular images of paperclip DNA triplexes formation in a buffer solution of pH 6.0 by atomic force microscopy (AFM). Meanwhile, a diffuse "tail" of unwound DNA was observed in pH 8.0 solution. This designable approach in visualizing the overall structures and shapes of oligo-DNAs at the single molecular level, by AFM, is applicable to other biopolymers as well.
AB - DNA triplex modulates gene expression by forming stable conformation in physiological condition. However, it is not feasible to observe this unique molecular structure of large molecule with 54 oligodeoxynucleotides directly by conventional nuclear magnetic approach. In this study, we observed directly single molecular images of paperclip DNA triplexes formation in a buffer solution of pH 6.0 by atomic force microscopy (AFM). Meanwhile, a diffuse "tail" of unwound DNA was observed in pH 8.0 solution. This designable approach in visualizing the overall structures and shapes of oligo-DNAs at the single molecular level, by AFM, is applicable to other biopolymers as well.
KW - Atomic force microscopy
KW - DNA triplex
KW - Single molecule image
KW - Tight-turn paperclip DNA
UR - http://www.scopus.com/inward/record.url?scp=70350565047&partnerID=8YFLogxK
U2 - 10.1007/s12010-008-8390-1
DO - 10.1007/s12010-008-8390-1
M3 - Article
C2 - 18931945
AN - SCOPUS:70350565047
SN - 0273-2289
VL - 159
SP - 261
EP - 269
JO - Applied Biochemistry and Biotechnology
JF - Applied Biochemistry and Biotechnology
IS - 1
ER -