Direct and in vitro observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling

C. Y. Cheng; E. Perevedentseva; J. S. Tu; P. H. Chung; C. L. Cheng; K. K. Liu; Jui-I Chao; P. H. Chen; Chia-Ching Chang

doi:10.1063/1.2727557

Direct and in vitro observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling

C. Y. Cheng^*, E. Perevedentseva, J. S. Tu, P. H. Chung, C. L. Cheng, K. K. Liu, Jui-I Chao, P. H. Chen, Chia-Ching Chang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

124 Scopus citations

Abstract

This letter presents direct observation of growth hormone receptor in one single cancer cell using nanodiamond-growth hormone complex as a specific probe. The interaction of surface growth hormone receptor of A549 human lung epithelial cells with growth hormone was observed using nanodiamond's unique spectroscopic signal via confocal Raman mapping. The growth hormone molecules were covalent conjugated to 100 nm diameter carboxylated nanodiamonds, which can be recognized specifically by the growth hormone receptors of A549 cell. The Raman spectroscopic signal of diamond provides direct and in vitro observation of growth hormone receptors in physiology condition in a single cell level.

Original language	English
Article number	163903
Journal	Applied Physics Letters
Volume	90
Issue number	16
DOIs	https://doi.org/10.1063/1.2727557
State	Published - 16 Apr 2007

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title = "Direct and in vitro observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling",

abstract = "This letter presents direct observation of growth hormone receptor in one single cancer cell using nanodiamond-growth hormone complex as a specific probe. The interaction of surface growth hormone receptor of A549 human lung epithelial cells with growth hormone was observed using nanodiamond's unique spectroscopic signal via confocal Raman mapping. The growth hormone molecules were covalent conjugated to 100 nm diameter carboxylated nanodiamonds, which can be recognized specifically by the growth hormone receptors of A549 cell. The Raman spectroscopic signal of diamond provides direct and in vitro observation of growth hormone receptors in physiology condition in a single cell level.",

author = "Cheng, {C. Y.} and E. Perevedentseva and Tu, {J. S.} and Chung, {P. H.} and Cheng, {C. L.} and Liu, {K. K.} and Jui-I Chao and Chen, {P. H.} and Chia-Ching Chang",

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doi = "10.1063/1.2727557",

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volume = "90",

journal = "Applied Physics Letters",

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T1 - Direct and in vitro observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling

AU - Cheng, C. Y.

AU - Perevedentseva, E.

AU - Tu, J. S.

AU - Chung, P. H.

AU - Cheng, C. L.

AU - Liu, K. K.

AU - Chao, Jui-I

AU - Chen, P. H.

AU - Chang, Chia-Ching

PY - 2007/4/16

Y1 - 2007/4/16

N2 - This letter presents direct observation of growth hormone receptor in one single cancer cell using nanodiamond-growth hormone complex as a specific probe. The interaction of surface growth hormone receptor of A549 human lung epithelial cells with growth hormone was observed using nanodiamond's unique spectroscopic signal via confocal Raman mapping. The growth hormone molecules were covalent conjugated to 100 nm diameter carboxylated nanodiamonds, which can be recognized specifically by the growth hormone receptors of A549 cell. The Raman spectroscopic signal of diamond provides direct and in vitro observation of growth hormone receptors in physiology condition in a single cell level.

AB - This letter presents direct observation of growth hormone receptor in one single cancer cell using nanodiamond-growth hormone complex as a specific probe. The interaction of surface growth hormone receptor of A549 human lung epithelial cells with growth hormone was observed using nanodiamond's unique spectroscopic signal via confocal Raman mapping. The growth hormone molecules were covalent conjugated to 100 nm diameter carboxylated nanodiamonds, which can be recognized specifically by the growth hormone receptors of A549 cell. The Raman spectroscopic signal of diamond provides direct and in vitro observation of growth hormone receptors in physiology condition in a single cell level.

UR - http://www.scopus.com/inward/record.url?scp=34247323524&partnerID=8YFLogxK

U2 - 10.1063/1.2727557

DO - 10.1063/1.2727557

M3 - Article

AN - SCOPUS:34247323524

SN - 0003-6951

VL - 90

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 16

M1 - 163903

ER -

Direct and in vitro observation of growth hormone receptor molecules in A549 human lung epithelial cells by nanodiamond labeling

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