Digital biosensor for human cerebrospinal fluid detection with single-use sensing strips

Minghan Xian*, Chan Wen Chiu, Patrick H. Carey, Chaker Fares, Liya Chen, Rena Wu, Fan Ren, Cheng Tse Tsai, Siang Sin Shan, Yu Te Liao, Josephine F. Esquivel-Upshaw, Stephen J. Pearton

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Leakage of human cerebrospinal fluid (CSF) caused by trauma or other reasons presents exceptional challenges in clinical analysis and can have severe medical repercussions. Conventional test methods, including enzyme-linked immunosorbent assay and immunofixation electrophoresis testing, typically are performed at a few clinical reference laboratories, which may potentially delay proper diagnosis and treatment. At the same time, medical imaging can serve as a secondary diagnosis tool. This work presented here reports the use of a point-of-care electrochemical sensor for detection of beta-2-transferrin (B2T), a unique isomer of transferrin that is present exclusively in human CSF but is absent in other bodily fluids. Limits of detection were examined via serial dilution of human samples with known B2T concentrations down to 7 × 10-12 g B2T/ml while maintaining excellent sensitivity. Nine human samples with varying levels of B2T were compared using up to 100 times dilution to confirm the validity of sensor output across different patient samples.

Original languageEnglish
Article number023202
JournalJournal of Vacuum Science and Technology B: Nanotechnology and Microelectronics
Issue number2
StatePublished - 1 Mar 2022


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