Differentiation of bacteria cell wall using Raman scattering enhanced by nanoparticle array

T. Y. Liu, Y. Chen, H. H. Wang, Y. L. Huang, Y. C. Chao, K. T. Tsai, W. C. Cheng, C. Y. Chuang, Y. H. Tsai, C. Y. Huang, D. W. Wang, C. H. Lin, J. K. Wang, Y. L. Wang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


We have fabricated surface-enhanced Raman scattering (SERS) substrates based on arrays of silver nanoparticles grown on porous anodic alumina templates. Using this nanotechnology platform, label-free and high-speed detection of bacteria are achieved. SERS spectra of various bacteria including Staphylococcus Aureus (Gram-positive bacterium), Klebsiella Pneumoniae (Gram-negative bacterium), and Mycobacterium Smegmatis (Mycobacterium) were recorded. The highly reproducible SERS-based technological platform is capable of differentiating different kinds of bacteria by PCA, LDA, clustering analysis, and SVM methods, which provides promising opportunity for biosensing of clinical microbes.

Original languageEnglish
Pages (from-to)5004-5008
Number of pages5
JournalJournal of Nanoscience and Nanotechnology
Issue number6
StatePublished - 2012


  • Bacteria
  • Classification
  • Nanoparticle Array
  • Surface-Enhanced Raman Scattering (SERS)


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