Differential network biology reveals a positive correlation between a novel protein-protein interaction and cancer cells migration

Chia Hung Liu; Tzu Chi Chen; Chun Houh Chen; Cheng Yan Kao; Chi Ying F. Huang

doi:10.1109/EMBC.2013.6610097

Differential network biology reveals a positive correlation between a novel protein-protein interaction and cancer cells migration

Chia Hung Liu
, Tzu Chi Chen
, Chun Houh Chen
, Cheng Yan Kao
, Chi Ying F. Huang^*

^*Corresponding author for this work

Institute of Biopharmaceutical Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

3 Scopus citations

Abstract

This paper introduces a differential network biology for discovering tumor migration. We applied statistical methods to prioritize PPI candidates and an in situ proximity ligation assay to verify 67 endogenous PPIs among 21 interlinked pathways in two hepatocellular carcinoma (HCC) cells, Huh7 (minimally migratory cells) and Mahlavu (highly migratory cells). Differential network biology analysis was applied to determine the novel interaction, CRKL-FLT1, has a high centrality ranking, and the expression of this interaction is strongly correlated with the migratory ability of HCC and other cancer cell lines. Knockdown of CRKL and FLT1 in HCC cells leads to a decrease in cell migration. This study demonstrated that functional exploration of a disease network with differential network in interlinked pathways via PPIs can be used to discover tumor migration.

Original language	English
Title of host publication	2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
Pages	2700-2703
Number of pages	4
DOIs	https://doi.org/10.1109/EMBC.2013.6610097
State	Published - 2013
Event	2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka, Japan Duration: 3 Jul 2013 → 7 Jul 2013

Publication series

Name	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)	1557-170X

Conference

Conference	2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
Country/Territory	Japan
City	Osaka
Period	3/07/13 → 7/07/13

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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10.1109/EMBC.2013.6610097

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Liu, C. H., Chen, T. C., Chen, C. H., Kao, C. Y., & Huang, C. Y. F. (2013). Differential network biology reveals a positive correlation between a novel protein-protein interaction and cancer cells migration. In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 (pp. 2700-2703). Article 6610097 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/EMBC.2013.6610097

Liu, Chia Hung ; Chen, Tzu Chi ; Chen, Chun Houh et al. / Differential network biology reveals a positive correlation between a novel protein-protein interaction and cancer cells migration. 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013. 2013. pp. 2700-2703 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

@inproceedings{b6e28d1b0ff1463ebb97883a442f3237,

title = "Differential network biology reveals a positive correlation between a novel protein-protein interaction and cancer cells migration",

abstract = "This paper introduces a differential network biology for discovering tumor migration. We applied statistical methods to prioritize PPI candidates and an in situ proximity ligation assay to verify 67 endogenous PPIs among 21 interlinked pathways in two hepatocellular carcinoma (HCC) cells, Huh7 (minimally migratory cells) and Mahlavu (highly migratory cells). Differential network biology analysis was applied to determine the novel interaction, CRKL-FLT1, has a high centrality ranking, and the expression of this interaction is strongly correlated with the migratory ability of HCC and other cancer cell lines. Knockdown of CRKL and FLT1 in HCC cells leads to a decrease in cell migration. This study demonstrated that functional exploration of a disease network with differential network in interlinked pathways via PPIs can be used to discover tumor migration.",

author = "Liu, \{Chia Hung\} and Chen, \{Tzu Chi\} and Chen, \{Chun Houh\} and Kao, \{Cheng Yan\} and Huang, \{Chi Ying F.\}",

year = "2013",

doi = "10.1109/EMBC.2013.6610097",

language = "English",

isbn = "9781457702167",

series = "Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS",

pages = "2700--2703",

booktitle = "2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013",

note = "2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 ; Conference date: 03-07-2013 Through 07-07-2013",

}

Liu, CH, Chen, TC, Chen, CH, Kao, CY & Huang, CYF 2013, Differential network biology reveals a positive correlation between a novel protein-protein interaction and cancer cells migration. in 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013., 6610097, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, pp. 2700-2703, 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013, Osaka, Japan, 3/07/13. https://doi.org/10.1109/EMBC.2013.6610097

Differential network biology reveals a positive correlation between a novel protein-protein interaction and cancer cells migration. / Liu, Chia Hung; Chen, Tzu Chi; Chen, Chun Houh et al.
2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013. 2013. p. 2700-2703 6610097 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Differential network biology reveals a positive correlation between a novel protein-protein interaction and cancer cells migration

AU - Liu, Chia Hung

AU - Chen, Tzu Chi

AU - Chen, Chun Houh

AU - Kao, Cheng Yan

AU - Huang, Chi Ying F.

PY - 2013

Y1 - 2013

N2 - This paper introduces a differential network biology for discovering tumor migration. We applied statistical methods to prioritize PPI candidates and an in situ proximity ligation assay to verify 67 endogenous PPIs among 21 interlinked pathways in two hepatocellular carcinoma (HCC) cells, Huh7 (minimally migratory cells) and Mahlavu (highly migratory cells). Differential network biology analysis was applied to determine the novel interaction, CRKL-FLT1, has a high centrality ranking, and the expression of this interaction is strongly correlated with the migratory ability of HCC and other cancer cell lines. Knockdown of CRKL and FLT1 in HCC cells leads to a decrease in cell migration. This study demonstrated that functional exploration of a disease network with differential network in interlinked pathways via PPIs can be used to discover tumor migration.

AB - This paper introduces a differential network biology for discovering tumor migration. We applied statistical methods to prioritize PPI candidates and an in situ proximity ligation assay to verify 67 endogenous PPIs among 21 interlinked pathways in two hepatocellular carcinoma (HCC) cells, Huh7 (minimally migratory cells) and Mahlavu (highly migratory cells). Differential network biology analysis was applied to determine the novel interaction, CRKL-FLT1, has a high centrality ranking, and the expression of this interaction is strongly correlated with the migratory ability of HCC and other cancer cell lines. Knockdown of CRKL and FLT1 in HCC cells leads to a decrease in cell migration. This study demonstrated that functional exploration of a disease network with differential network in interlinked pathways via PPIs can be used to discover tumor migration.

UR - https://www.scopus.com/pages/publications/84886475018

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DO - 10.1109/EMBC.2013.6610097

M3 - Conference contribution

C2 - 24110284

AN - SCOPUS:84886475018

SN - 9781457702167

T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS

SP - 2700

EP - 2703

BT - 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013

T2 - 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013

Y2 - 3 July 2013 through 7 July 2013

ER -

Liu CH, Chen TC, Chen CH, Kao CY, Huang CYF. Differential network biology reveals a positive correlation between a novel protein-protein interaction and cancer cells migration. In 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013. 2013. p. 2700-2703. 6610097. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). doi: 10.1109/EMBC.2013.6610097

Differential network biology reveals a positive correlation between a novel protein-protein interaction and cancer cells migration

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