Differential effects of nonylphenol on testosterone secretion in rat Leydig cells

Jen Jung Wu; Kai Lee Wang; Shyi Wu Wang; Guey Shyang Hwang; I. Fang Mao; Mei Lien Chen; Paulus S. Wang

doi:10.1016/j.tox.2009.10.030

Differential effects of nonylphenol on testosterone secretion in rat Leydig cells

Jen Jung Wu, Kai Lee Wang, Shyi Wu Wang, Guey Shyang Hwang, I. Fang Mao, Mei Lien Chen, Paulus S. Wang^*

^*Corresponding author for this work

Institute of Environmental and Occupational Health Sciences

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Nonylphenol (NP), a final metabolite of nonylphenol polyethoxylate, has been reported to interfere with male reproduction. However, its mechanisms are not fully understood. In the present study, we examined the effects of NP on steroidogenesis of testosterone in rat Leydig cells. The testosterone concentrations in rat plasma were examined after intravenous injection of NP (100 μg/kg) at different time intervals. In addition, rat Leydig cells were challenged with different concentrations of NP (4.25-127.5 μM) to evaluate its influences on testosterone steroidogenesis. Administration of NP showed a decrease of hCG-induced plasma testosterone. Moreover, in vitro experiments revealed that NP (127.5 μM) alone stimulated testosterone release through increase of both protein levels and activities of the StAR and P450_SCC. In contrast, NP inhibited hCG-induced testosterone release in rat Leydig cells. The inhibitory effect was also observed after incubation of the Leydig cells in the presence of different precursors. These results suggested that NP had differential effects on testosterone synthesis.

Original language	English
Pages (from-to)	1-7
Number of pages	7
Journal	Toxicology
Volume	268
Issue number	1-2
DOIs	https://doi.org/10.1016/j.tox.2009.10.030
State	Published - 31 Jan 2010

Keywords

Cyclic AMP
Nonylphenol
P450
StAR
Steroidogenesis
Testosterone

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10.1016/j.tox.2009.10.030

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title = "Differential effects of nonylphenol on testosterone secretion in rat Leydig cells",

abstract = "Nonylphenol (NP), a final metabolite of nonylphenol polyethoxylate, has been reported to interfere with male reproduction. However, its mechanisms are not fully understood. In the present study, we examined the effects of NP on steroidogenesis of testosterone in rat Leydig cells. The testosterone concentrations in rat plasma were examined after intravenous injection of NP (100 μg/kg) at different time intervals. In addition, rat Leydig cells were challenged with different concentrations of NP (4.25-127.5 μM) to evaluate its influences on testosterone steroidogenesis. Administration of NP showed a decrease of hCG-induced plasma testosterone. Moreover, in vitro experiments revealed that NP (127.5 μM) alone stimulated testosterone release through increase of both protein levels and activities of the StAR and P450SCC. In contrast, NP inhibited hCG-induced testosterone release in rat Leydig cells. The inhibitory effect was also observed after incubation of the Leydig cells in the presence of different precursors. These results suggested that NP had differential effects on testosterone synthesis.",

keywords = "Cyclic AMP, Nonylphenol, P450, StAR, Steroidogenesis, Testosterone",

author = "Wu, {Jen Jung} and Wang, {Kai Lee} and Wang, {Shyi Wu} and Hwang, {Guey Shyang} and Mao, {I. Fang} and Chen, {Mei Lien} and Wang, {Paulus S.}",

note = "Funding Information: We thank Dr. Douglas M. Stocco, Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX, for providing the anti-StAR antibody. Also, we thank Dr. Bon-Chu Chung, Academia Sinica, Taipei, Taiwan, Republic of China for providing anti-P450 SCC antibody. This work was supported by grants ( NSC93-2621-Z-010-003 and NSC94-2621-Z-010-003 ) from the National Science Council, Taiwan, R.O.C. ",

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AU - Wu, Jen Jung

AU - Wang, Kai Lee

AU - Wang, Shyi Wu

AU - Hwang, Guey Shyang

AU - Mao, I. Fang

AU - Chen, Mei Lien

AU - Wang, Paulus S.

N1 - Funding Information: We thank Dr. Douglas M. Stocco, Department of Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, Lubbock, TX, for providing the anti-StAR antibody. Also, we thank Dr. Bon-Chu Chung, Academia Sinica, Taipei, Taiwan, Republic of China for providing anti-P450 SCC antibody. This work was supported by grants ( NSC93-2621-Z-010-003 and NSC94-2621-Z-010-003 ) from the National Science Council, Taiwan, R.O.C.

PY - 2010/1/31

Y1 - 2010/1/31

N2 - Nonylphenol (NP), a final metabolite of nonylphenol polyethoxylate, has been reported to interfere with male reproduction. However, its mechanisms are not fully understood. In the present study, we examined the effects of NP on steroidogenesis of testosterone in rat Leydig cells. The testosterone concentrations in rat plasma were examined after intravenous injection of NP (100 μg/kg) at different time intervals. In addition, rat Leydig cells were challenged with different concentrations of NP (4.25-127.5 μM) to evaluate its influences on testosterone steroidogenesis. Administration of NP showed a decrease of hCG-induced plasma testosterone. Moreover, in vitro experiments revealed that NP (127.5 μM) alone stimulated testosterone release through increase of both protein levels and activities of the StAR and P450SCC. In contrast, NP inhibited hCG-induced testosterone release in rat Leydig cells. The inhibitory effect was also observed after incubation of the Leydig cells in the presence of different precursors. These results suggested that NP had differential effects on testosterone synthesis.

AB - Nonylphenol (NP), a final metabolite of nonylphenol polyethoxylate, has been reported to interfere with male reproduction. However, its mechanisms are not fully understood. In the present study, we examined the effects of NP on steroidogenesis of testosterone in rat Leydig cells. The testosterone concentrations in rat plasma were examined after intravenous injection of NP (100 μg/kg) at different time intervals. In addition, rat Leydig cells were challenged with different concentrations of NP (4.25-127.5 μM) to evaluate its influences on testosterone steroidogenesis. Administration of NP showed a decrease of hCG-induced plasma testosterone. Moreover, in vitro experiments revealed that NP (127.5 μM) alone stimulated testosterone release through increase of both protein levels and activities of the StAR and P450SCC. In contrast, NP inhibited hCG-induced testosterone release in rat Leydig cells. The inhibitory effect was also observed after incubation of the Leydig cells in the presence of different precursors. These results suggested that NP had differential effects on testosterone synthesis.

KW - Cyclic AMP

KW - Nonylphenol

KW - P450

KW - StAR

KW - Steroidogenesis

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Differential effects of nonylphenol on testosterone secretion in rat Leydig cells

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