Dietary flavonoids luteolin and quercetin suppressed cancer stem cell properties and metastatic potential of isolated prostate cancer cells

Pei Hsun Tsai; Chia Hsiung Cheng; Chun Yu Lin; Ying Tang Huang; Lung Ta Lee; Chithan C. Kandaswami; Yo Chuen Lin; Kevin Po Hao Lee; Chin Chun Hung; Jiuan Jiuan Hwang; Ferng Chun Ke; Geen Dong Chang; Ming Ting Lee

doi:10.21873/anticanres.11234

Dietary flavonoids luteolin and quercetin suppressed cancer stem cell properties and metastatic potential of isolated prostate cancer cells

Pei Hsun Tsai, Chia Hsiung Cheng, Chun Yu Lin, Ying Tang Huang, Lung Ta Lee, Chithan C. Kandaswami, Yo Chuen Lin, Kevin Po Hao Lee, Chin Chun Hung, Jiuan Jiuan Hwang, Ferng Chun Ke, Geen Dong Chang, Ming Ting Lee^*

^*Corresponding author for this work

Institute of Physiology

Research output: Contribution to journal › Article › peer-review

58 Scopus citations

Abstract

A highly invasive Du145-III subline was isolated by three successive passages of the parental Du145 prostate tumor cell line (Du145-P) through a Boyden chamber with matrigel-coated membrane support. Du145-III cells showed great invasion potential based on their increased ability to spread/migrate and enhanced expression/secretion of the matrix metalloproteinase 9 (MMP9). Du145-III cells exerted vasculogenic mimicry (VM) properties, reminiscent of endothelial cell characteristics and expressed elevated levels of cancer stem cell (CSC) markers, including Nanog, Sox2, CD44 and ABCG2 and ability to self-renew. Of prominence, MMP9 was required for the induction of VM and for increased stemness in Du145-III cells. Using Du145-III as a model, the effects of dietary flavonoids, luteolin and quercetin, were evaluated on stemness and invasion capacity of Du145-III cells in relation to JNK signaling pathway activation. These flavonoids depressed the malignancy of highly invasive Du145-III cells, VM, anchorage-independent spheroid formation and expression of certain CSC markers. Since luteolin and quercetin were able to target CSC cells and prevent cancer cell invasiveness, may serve as potential anti-angiogenesis and anti-metastasis agents.

Original language	English
Pages (from-to)	6367-6380
Number of pages	14
Journal	Anticancer Research
Volume	36
Issue number	12
DOIs	https://doi.org/10.21873/anticanres.11234
State	Published - Dec 2016

Keywords

Cancer stem cell
Luteolin
Metastasis
Prostate cancer
Quercetin
Vasculogenic mimicry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.21873/anticanres.11234

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Tsai, P. H., Cheng, C. H., Lin, C. Y., Huang, Y. T., Lee, L. T., Kandaswami, C. C., Lin, Y. C., Lee, K. P. H., Hung, C. C., Hwang, J. J., Ke, F. C., Chang, G. D., & Lee, M. T. (2016). Dietary flavonoids luteolin and quercetin suppressed cancer stem cell properties and metastatic potential of isolated prostate cancer cells. Anticancer Research, 36(12), 6367-6380. https://doi.org/10.21873/anticanres.11234

@article{1beccb0d05014faa9673ac726a051e63,

title = "Dietary flavonoids luteolin and quercetin suppressed cancer stem cell properties and metastatic potential of isolated prostate cancer cells",

abstract = "A highly invasive Du145-III subline was isolated by three successive passages of the parental Du145 prostate tumor cell line (Du145-P) through a Boyden chamber with matrigel-coated membrane support. Du145-III cells showed great invasion potential based on their increased ability to spread/migrate and enhanced expression/secretion of the matrix metalloproteinase 9 (MMP9). Du145-III cells exerted vasculogenic mimicry (VM) properties, reminiscent of endothelial cell characteristics and expressed elevated levels of cancer stem cell (CSC) markers, including Nanog, Sox2, CD44 and ABCG2 and ability to self-renew. Of prominence, MMP9 was required for the induction of VM and for increased stemness in Du145-III cells. Using Du145-III as a model, the effects of dietary flavonoids, luteolin and quercetin, were evaluated on stemness and invasion capacity of Du145-III cells in relation to JNK signaling pathway activation. These flavonoids depressed the malignancy of highly invasive Du145-III cells, VM, anchorage-independent spheroid formation and expression of certain CSC markers. Since luteolin and quercetin were able to target CSC cells and prevent cancer cell invasiveness, may serve as potential anti-angiogenesis and anti-metastasis agents.",

keywords = "Cancer stem cell, Luteolin, Metastasis, Prostate cancer, Quercetin, Vasculogenic mimicry",

author = "Tsai, {Pei Hsun} and Cheng, {Chia Hsiung} and Lin, {Chun Yu} and Huang, {Ying Tang} and Lee, {Lung Ta} and Kandaswami, {Chithan C.} and Lin, {Yo Chuen} and Lee, {Kevin Po Hao} and Hung, {Chin Chun} and Hwang, {Jiuan Jiuan} and Ke, {Ferng Chun} and Chang, {Geen Dong} and Lee, {Ming Ting}",

note = "Funding Information: This work was supported in part by grants from the National Science Council of Taiwan (NSC 102-2311-B-002-046-MY3 to G.D.C.); Ministry of Science and Technology, Taiwan , R.O.C. (104-2311-B-038-001 to C.H.C.) and the Taiwan Academia Sinica Thematic Project (AS-96-TPB06 to M.T.L.)",

year = "2016",

month = dec,

doi = "10.21873/anticanres.11234",

language = "English",

volume = "36",

pages = "6367--6380",

journal = "Anticancer Research",

issn = "0250-7005",

publisher = "International Institute of Anticancer Research",

number = "12",

}

Tsai, PH, Cheng, CH, Lin, CY, Huang, YT, Lee, LT, Kandaswami, CC, Lin, YC, Lee, KPH, Hung, CC, Hwang, JJ, Ke, FC, Chang, GD & Lee, MT 2016, 'Dietary flavonoids luteolin and quercetin suppressed cancer stem cell properties and metastatic potential of isolated prostate cancer cells', Anticancer Research, vol. 36, no. 12, pp. 6367-6380. https://doi.org/10.21873/anticanres.11234

TY - JOUR

T1 - Dietary flavonoids luteolin and quercetin suppressed cancer stem cell properties and metastatic potential of isolated prostate cancer cells

AU - Tsai, Pei Hsun

AU - Cheng, Chia Hsiung

AU - Lin, Chun Yu

AU - Huang, Ying Tang

AU - Lee, Lung Ta

AU - Kandaswami, Chithan C.

AU - Lin, Yo Chuen

AU - Lee, Kevin Po Hao

AU - Hung, Chin Chun

AU - Hwang, Jiuan Jiuan

AU - Ke, Ferng Chun

AU - Chang, Geen Dong

AU - Lee, Ming Ting

N1 - Funding Information: This work was supported in part by grants from the National Science Council of Taiwan (NSC 102-2311-B-002-046-MY3 to G.D.C.); Ministry of Science and Technology, Taiwan , R.O.C. (104-2311-B-038-001 to C.H.C.) and the Taiwan Academia Sinica Thematic Project (AS-96-TPB06 to M.T.L.)

PY - 2016/12

Y1 - 2016/12

N2 - A highly invasive Du145-III subline was isolated by three successive passages of the parental Du145 prostate tumor cell line (Du145-P) through a Boyden chamber with matrigel-coated membrane support. Du145-III cells showed great invasion potential based on their increased ability to spread/migrate and enhanced expression/secretion of the matrix metalloproteinase 9 (MMP9). Du145-III cells exerted vasculogenic mimicry (VM) properties, reminiscent of endothelial cell characteristics and expressed elevated levels of cancer stem cell (CSC) markers, including Nanog, Sox2, CD44 and ABCG2 and ability to self-renew. Of prominence, MMP9 was required for the induction of VM and for increased stemness in Du145-III cells. Using Du145-III as a model, the effects of dietary flavonoids, luteolin and quercetin, were evaluated on stemness and invasion capacity of Du145-III cells in relation to JNK signaling pathway activation. These flavonoids depressed the malignancy of highly invasive Du145-III cells, VM, anchorage-independent spheroid formation and expression of certain CSC markers. Since luteolin and quercetin were able to target CSC cells and prevent cancer cell invasiveness, may serve as potential anti-angiogenesis and anti-metastasis agents.

AB - A highly invasive Du145-III subline was isolated by three successive passages of the parental Du145 prostate tumor cell line (Du145-P) through a Boyden chamber with matrigel-coated membrane support. Du145-III cells showed great invasion potential based on their increased ability to spread/migrate and enhanced expression/secretion of the matrix metalloproteinase 9 (MMP9). Du145-III cells exerted vasculogenic mimicry (VM) properties, reminiscent of endothelial cell characteristics and expressed elevated levels of cancer stem cell (CSC) markers, including Nanog, Sox2, CD44 and ABCG2 and ability to self-renew. Of prominence, MMP9 was required for the induction of VM and for increased stemness in Du145-III cells. Using Du145-III as a model, the effects of dietary flavonoids, luteolin and quercetin, were evaluated on stemness and invasion capacity of Du145-III cells in relation to JNK signaling pathway activation. These flavonoids depressed the malignancy of highly invasive Du145-III cells, VM, anchorage-independent spheroid formation and expression of certain CSC markers. Since luteolin and quercetin were able to target CSC cells and prevent cancer cell invasiveness, may serve as potential anti-angiogenesis and anti-metastasis agents.

KW - Cancer stem cell

KW - Luteolin

KW - Metastasis

KW - Prostate cancer

KW - Quercetin

KW - Vasculogenic mimicry

UR - http://www.scopus.com/inward/record.url?scp=85002487118&partnerID=8YFLogxK

U2 - 10.21873/anticanres.11234

DO - 10.21873/anticanres.11234

M3 - Article

C2 - 27919958

AN - SCOPUS:85002487118

SN - 0250-7005

VL - 36

SP - 6367

EP - 6380

JO - Anticancer Research

JF - Anticancer Research

IS - 12

ER -

Dietary flavonoids luteolin and quercetin suppressed cancer stem cell properties and metastatic potential of isolated prostate cancer cells

Abstract

Keywords

UN SDGs

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