Development of Stereo NIR-II Fluorescence Imaging System for 3D Tumor Vasculature in Small Animals

Shih Po Su, Syue-Liang Lin, Yang-Hsiang Chan, Yi Jang Lee, Yun Chen Lee, Pin Xuan Zeng, Yi Xuan Li, Muh-Hwa Yang, Hui-Hua Chiang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


Near-infrared-II (NIR-II, 1000–1700 nm) fluorescence imaging boasts high spatial resolution and deep tissue penetration due to low light scattering, reduced photon absorption, and low tissue autofluorescence. NIR-II biological imaging is applied mainly in the noninvasive visualiza-tion of blood vessels and tumors in deep tissue. In the study, a stereo NIR-II fluorescence imaging system was developed for acquiring three-dimension (3D) images on tumor vasculature in real-time, on top of the development of fluorescent semiconducting polymer dots (IR-TPE Pdots) with ultra-bright NIR-II fluorescence (1000–1400 nm) and high stability to perform long-term fluorescence imaging. The NIR-II imaging system only consists of one InGaAs camera and a moving stage to simulate left-eye view and right-eye view for the construction of 3D in-depth blood vessel images. The system was validated with blood vessel phantom of tumor-bearing mice and was applied suc-cessfully in obtaining 3D blood vessel images with 0.6 mm-and 5 mm-depth resolution and 0.15 mm spatial resolution. The NIR-II stereo vision provides precise 3D information on the tumor mi-croenvironment and blood vessel path.

Original languageEnglish
Article number85
Issue number2
StatePublished - Feb 2022


  • NIR-II fluorescence imaging
  • Polymer dots
  • Reconstruction
  • Stereo imaging
  • Tumor vas-culature


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