TY - JOUR
T1 - Determining early adhesion of cells on polysaccharides/PCL surfaces by a quartz crystal microbalance
AU - Chung, Tze Wen
AU - Tyan, Yu Chang
AU - Lee, Rong Ho
AU - Ho, Chia Wei
N1 - Funding Information:
Acknowledgments The authors would like to thank the National Science Council of the Republic of China, Taiwan, under project No.NSC-101-2221-E-224-011-MY2 for financially supporting this research. NSYSUKMU 101-015 research project is also acknowledged. Ted Knoy is appreciated for his editorial assistance.
PY - 2012/12
Y1 - 2012/12
N2 - The early adhesions of cells to various biopolymers are important to their growths and proliferations. Here, the adhesion of cells (e.g., fibroblasts) on the electrode of a quartz crystal microbalance (QCM) that was coated by PCL or PEG/ PCL and further adsorbed by chitosan (CS) or CS/hyaluronic acid (HA) layers, was examined by cell-counting technique, QCMmethod and MTS assay under a serum-free condition for 3 h. The surfaces on electrodes of the QCM were confirmed to have been modified by measuring their contact angles, FT-IR spectra and the weights of biopolymers affected the frequency shifts of the QCM. Among tested surfaces on electrodes, the adhesion of fibroblasts on a HA/CS/PCL surface was the most (e.g., 3.08 × 105 cells/cm2) while that on a PEG/PCL surface was the least (e.g., 0.7 × 105 cells/cm2), as determined by cell-counting technique. The frequency shift and the mass of adhering fibroblasts on HA/CS/PCL electrodes were -3,537 ± 770 Hz and 3.78 ± 0.22 μg (n = 3), respectively, that were significantly exceeded those on other electrodes (-393 ± 58 Hz and 0.32 ± 0.06 μg, n = 3, respectively, for PEG/PCL electrodes). These results were consistent with cellcounting technique. Although MTS assay yielded similar results, it was less sensitive than the two aforementioned methods. In conclusion, modified electrodes of aQCMprovide a convenient and sensitive method for examining the early adhesion of cells (e.g., 3 h) to biopolymer surfaces.
AB - The early adhesions of cells to various biopolymers are important to their growths and proliferations. Here, the adhesion of cells (e.g., fibroblasts) on the electrode of a quartz crystal microbalance (QCM) that was coated by PCL or PEG/ PCL and further adsorbed by chitosan (CS) or CS/hyaluronic acid (HA) layers, was examined by cell-counting technique, QCMmethod and MTS assay under a serum-free condition for 3 h. The surfaces on electrodes of the QCM were confirmed to have been modified by measuring their contact angles, FT-IR spectra and the weights of biopolymers affected the frequency shifts of the QCM. Among tested surfaces on electrodes, the adhesion of fibroblasts on a HA/CS/PCL surface was the most (e.g., 3.08 × 105 cells/cm2) while that on a PEG/PCL surface was the least (e.g., 0.7 × 105 cells/cm2), as determined by cell-counting technique. The frequency shift and the mass of adhering fibroblasts on HA/CS/PCL electrodes were -3,537 ± 770 Hz and 3.78 ± 0.22 μg (n = 3), respectively, that were significantly exceeded those on other electrodes (-393 ± 58 Hz and 0.32 ± 0.06 μg, n = 3, respectively, for PEG/PCL electrodes). These results were consistent with cellcounting technique. Although MTS assay yielded similar results, it was less sensitive than the two aforementioned methods. In conclusion, modified electrodes of aQCMprovide a convenient and sensitive method for examining the early adhesion of cells (e.g., 3 h) to biopolymer surfaces.
UR - http://www.scopus.com/inward/record.url?scp=84871428887&partnerID=8YFLogxK
U2 - 10.1007/s10856-012-4764-6
DO - 10.1007/s10856-012-4764-6
M3 - Article
C2 - 22968597
AN - SCOPUS:84871428887
SN - 0957-4530
VL - 23
SP - 3067
EP - 3073
JO - Journal of Materials Science: Materials in Medicine
JF - Journal of Materials Science: Materials in Medicine
IS - 12
ER -