Determination of three-dimensional molecular orientation of type-I collagen by circularly-polarized second harmonic generation imaging

Guan Yu Zhuo, Wei Han Hung, Fu Jen Kao

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

The content of collagen is up to 30% existing in mammals. It supports the main component of connective tissues such as skin, ligament, and cartilage. Among various types of collagen, type-I collagen is of the most abundance and has been broadly studied due to the importance in bioscience. Second harmonic generation (SHG) microscopy is an effective tool used to study the collagen organization without labeling. In this study, we used circular polarization instead of linear polarization to retrieve three-dimensional (3D) molecular orientation of type-I collagen with only two cross polarized SHG images without acquiring an image stack of varying polarization.

Original languageEnglish
Title of host publicationBiomedical Imaging and Sensing Conference
EditorsYasuhiro Awatsuji, Osamu Matoba, Yoshihisa Aizu, Toyohiko Yatagai
PublisherSPIE
ISBN (Electronic)9781510610033
DOIs
StatePublished - 2017
Event3rd Biomedical Imaging and Sensing Conference - Yokohama, Japan
Duration: 19 Apr 201721 Apr 2017

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume10251
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

Conference3rd Biomedical Imaging and Sensing Conference
Country/TerritoryJapan
CityYokohama
Period19/04/1721/04/17

Keywords

  • Chirality
  • Circular Dichroism
  • Collagen
  • Molecular Orientation
  • Second Harmonic Generation Microscopy

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