Detection of tetracycline using isothermal amplification

Chia Cheng Wu, Li An Wu, Yih Fan Chen, Lu Ting Chou

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

PCR is an important biosensing method since it can amplify DNA by many orders of magnitude. However, for food safety and environmental monitoring, the target molecule is usually not DNA. Here we demonstrate a detection method that integrates competitive assay format, magnetic bead separation, and isothermal DNA amplification for sensitive detection of tetracycline (TET) and other molecules. While TET is an antibiotic rather than oligonucleotide, our method allows the signal of TET detection to be amplified like conventional PCR. In addition, since we use isothermal amplification instead of conventional PCR, the temperature of the sample solution only needs to be maintained at a fixed temperature during the amplification process. We have successfully used the method to detect 10 pM TET. In addition, to improve the sensitivity of detection further, besides using EvaGreen dyes to quantify the amplified DNA, we have also tested another DNA quantification method that uses graphene oxide and a fluorescent DNA probe to increase the signal to background ratio of fluorescence detection.

Original languageEnglish
Title of host publication2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages210-213
Number of pages4
ISBN (Electronic)9781509030590
DOIs
StatePublished - 25 Aug 2017
Event12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017 - Los Angeles, United States
Duration: 9 Apr 201712 Apr 2017

Publication series

Name2017 IEEE 12th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017

Conference

Conference12th IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2017
Country/TerritoryUnited States
CityLos Angeles
Period9/04/1712/04/17

Keywords

  • DNA
  • isothermal amplification
  • PCR
  • tetracycline

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