Design and fabrication of a polyimide-based microelectrode array: Application in neural recording and repeatable electrolytic lesion in rat brain

You Yin Chen*, Hsin Yi Lai, Sheng Huang Lin, Chien Wen Cho, Wen Hung Chao, Chia Hsin Liao, Siny Tsang, Yi Fan Chen, Si Yue Lin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

84 Scopus citations

Abstract

The design and testing of a new microelectrode array, the NCTU (National Chiao Tung University) probe, was presented. Evaluation results showed it has good biocompatibility, high signal-to-noise ratio (SNR: the root mean square of background noise to the average peak-to-peak amplitude of spikes) during chronic neural recordings, and high reusability for electrolytic lesions. The probe was a flexible, polyimide-based microelectrode array with a long shaft (14.9 mm in length) and 16 electrodes (5 μm-thick and 16 μm in radius); its performance in chronic in vivo recordings was examined in rodents. To improve the precision of implantation, a metallic, impact-resistant layer was sandwiched between the polyimide layers to strengthen the probe. The three-dimensional (3D) structure of electrodes fabricated by electroplating produced rough textures that increased the effective surface area. The in vitro impedance of electrodes on the NCTU probe was 2.4 ± 0.52 MΩ at 1 kHz. In addition, post-surgical neural recordings of implanted NCTU probes were conducted for up to 40 days in awake, normally behaving rats. The electrodes on the NCTU probe functioned well and had a high SNR (range: 4-5) with reliable in vivo impedance (<0.7 MΩ). The electrodes were also robust enough to functionally record events, even after the anodal current (30 μA, 10 s) was repeatedly applied for 60 times. With good biocompatibility, high and stable SNR for chronic recording, and high tolerance for electrolytic lesion, the NCTU probe would serve as a useful device in future neuroscience research.

Original languageEnglish
Pages (from-to)6-16
Number of pages11
JournalJournal of Neuroscience Methods
Volume182
Issue number1
DOIs
StatePublished - 30 Aug 2009

Keywords

  • Chronic recording
  • Electrolytic lesion
  • Electroplating
  • Microelectrode array
  • Polyimide

Fingerprint

Dive into the research topics of 'Design and fabrication of a polyimide-based microelectrode array: Application in neural recording and repeatable electrolytic lesion in rat brain'. Together they form a unique fingerprint.

Cite this