Description of the transcriptomes of immune response-activated hemocytes from the mosquito vectors Aedes aegypti and Armigeres subalbatus

Lyric C. Bartholomay, Wen Long Cho, Thomas A. Rocheleau, Jon P. Boyle, Eric T. Beck, Jeremy F. Fuchs, Paul Liss, Michael Rusch, Katherine M. Butler, Roy Chen Chih Wu, Shih Pei Lin, Hang Yen Kuo, I. Yu Tsao, Chiung Yin Huang, Tze Tze Liu, Kwang Jen Hsiao, Shih Feng Tsai, Ueng Cheng Yang, Anthony J. Nappi, Nicole T. PernaChen Cheng Chen, Bruce M. Christensen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

107 Scopus citations

Abstract

Mosquito-borne diseases, including dengue, malaria, and lymphatic filariasis, exact a devastating toll on global health and economics, killing or debilitating millions every year (54). Mosquito innate immune responses are at the forefront of concerted research efforts aimed at defining potential target genes that could be manipulated to engineer pathogen resistance in vector populations. We aimed to describe the pivotal role that circulating blood cells (called hemocytes) play in immunity by generating a total of 11,952 Aedes aegypti and 12,790 Armigeres subalbatus expressed sequence tag (EST) sequences from immune response-activated hemocyte libraries. These ESTs collapsed into 2,686 and 2,107 EST clusters, respectively. The clusters were used to adapt the web-based interface for annotating bacterial genomes called A Systematic Annotation Package for Community Analysis of Genomes (ASAP) for analysis of ESTs. Each cluster was categorically characterized and annotated in ASAP based on sequence similarity to five sequence databases. The sequence data and annotations can be viewed in ASAP at https://asap.ahabs.wisc.edu/annotation/php/ ASAP1.htm. The data presented here represent the results of the first high-throughput in vivo analysis of the transcriptome of immunocytes from an invertebrate. Among the sequences are those for numerous immunity-related genes, many of which parallel those employed in vertebrate innate immunity, that have never been described for these mosquitoes.

Original languageEnglish
Pages (from-to)4114-4126
Number of pages13
JournalInfection and Immunity
Volume72
Issue number7
DOIs
StatePublished - Jul 2004

Fingerprint

Dive into the research topics of 'Description of the transcriptomes of immune response-activated hemocytes from the mosquito vectors Aedes aegypti and Armigeres subalbatus'. Together they form a unique fingerprint.

Cite this