Deep Tissue Multiphoton Microscopy Based on Advanced Femtosecond Fiber Sources

Shih Hsuan Chia, Hsiang Yu Chung, Wei Liu, Franz X. Kartner, Guoqing Chang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

The use of multiphoton microscopy has proven its potential on deep tissue virtual biopsy for early diagnosis and neuronal processes imaging. However, the capability of multiphoton microscopy strongly depends on the availability of easily operated femtosecond laser sources. By precise management of fiber-optic nonlinearity, we have demonstrated the way to generate nearly transform-limited femtosecond sources with the tunable wavelength range of 0.9 μm - 1.2 μm, covering both the GFP two-photon-excitation band and transparency window of common bio-tissues. The demonstrated approach is fully energy scalable to sub-100 nJ level of pulse energy with the spectral tunable range down to 1.31 μm, which paves the way to capture deep-tissue imaging contrast from third harmonic generation and three-photon-excited GFP fluorescence. We applied such an energetic source to a proof-of-principle study of ex vivo human skin based on harmonics (i.e., second-harmonic generation and third-harmonic generation) imaging. This new type of fiber-format energetic ultrafast source will be performed as a robust solution for multiphoton microscopy applications, as well as studying the damage assessments from thermal heating and multiphoton absorption during the imaging process.

Original languageEnglish
Title of host publication2018 20th International Conference on Transparent Optical Networks, ICTON 2018
PublisherIEEE Computer Society
ISBN (Electronic)9781538666043
DOIs
StatePublished - 26 Sep 2018
Event20th International Conference on Transparent Optical Networks, ICTON 2018 - Bucharest, Romania
Duration: 1 Jul 20185 Jul 2018

Publication series

NameInternational Conference on Transparent Optical Networks
Volume2018-July
ISSN (Electronic)2162-7339

Conference

Conference20th International Conference on Transparent Optical Networks, ICTON 2018
Country/TerritoryRomania
CityBucharest
Period1/07/185/07/18

Keywords

  • femtosecond fiber laser
  • multiphoton microscopy
  • nonlinear fiber optics.

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