Cultivation of Chlorella sp. GD using piggery wastewater for biomass and lipid production

Chiu Mei Kuo, Tsai Yu Chen, Tsung Hsien Lin, Chien Ya Kao, Jinn Tsyy Lai, Jo Shu Chang, Chih-Sheng Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

96 Scopus citations


The development of a culture system for Chlorella sp. GD to efficiently produce biomass and oil for biodiesel production was investigated. Chlorella sp. GD was cultivated with 0%, 25%, 50%, 75% and 100% piggery wastewater (diluted by medium) at 300μmolm-2s-1, a 2% CO2 aeration rate of 0.2vvm and 26±1°C; after a 10-day culture in batch cultures, the maximum specific growth rate and biomass productivity of the microalga obtained in 100% piggery wastewater were 0.839d-1 and 0.681gL-1d-1, respectively. The highest lipid content and lipid productivity were 29.3% and 0.155gL-1d-1 at 25% wastewater, respectively. In semi-continuous cultures, the biomass and lipid productivities with 25-75% wastewater ratios were greater than 0.852 and 0.128gL-1d-1, respectively. These results show that Chlorella sp. GD grows efficiently in piggery wastewater, and that a stable growth performance was achieved for long-term microalgal cultivation in a semi-continuous culture.

Original languageEnglish
Pages (from-to)326-333
Number of pages8
JournalBioresource Technology
StatePublished - 1 Oct 2015


  • Biomass
  • Lipid
  • Microalgae
  • Semi-continuous culture
  • Wastewater


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