Cucumarioside A 2 -2 causes macrophage activation in mouse spleen

Evgeny A. Pislyagin; Igor V. Manzhulo; Tatiana Y. Gorpenchenko; Pavel S. Dmitrenok; Sergey A. Avilov; Alexandra S. Silchenko; Yun-Ming Wang; Dmitry L. Aminin

doi:10.3390/md15110341

Cucumarioside A ₂ -2 causes macrophage activation in mouse spleen

Evgeny A. Pislyagin^*, Igor V. Manzhulo, Tatiana Y. Gorpenchenko, Pavel S. Dmitrenok, Sergey A. Avilov, Alexandra S. Silchenko, Yun-Ming Wang, Dmitry L. Aminin

^*Corresponding author for this work

Institute of Molecular Medicine and Bioengineering

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The immunomodulatory effect of triterpene glycoside cucumarioside A ₂ -2 (CA ₂ -2), isolated from the Far Eastern sea cucumber Cucumaria japonica, was compared with lipopolysaccharide (LPS) on mouse spleen. It has been shown that the intraperitoneal (i.p.) glycoside administration leads to increased spleen macrophage activating markers iba-1, IL-1β, iNOs, ROS and NO formation, with additional change of macrophage phenotype to M1. The mass spectrometry profiles of peptide/protein were obtained using MALDI-TOF-MS on the different parts of spleen sections isolated by laser mircodissection techniques. It was found that i.p. stimulation of animals with CA ₂ -2 leads to marked changes in the intensity of the characteristic peaks of spleen peptides/proteins, primarily in red pulp.

Original language	English
Article number	341
Number of pages	15
Journal	Marine Drugs
Volume	15
Issue number	11
DOIs	https://doi.org/10.3390/md15110341
State	Published - 1 Nov 2017

Keywords

IL-1β
Immunomodulation
LPS
NO
ROS
Sea cucumbers
Spleen
Triterpene glycosides
iNOs
iba-1

Access to Document

10.3390/md15110341

Cite this

@article{9aa8d7053bc94d66aedce94e71e13c0c,

title = "Cucumarioside A 2 -2 causes macrophage activation in mouse spleen",

abstract = " The immunomodulatory effect of triterpene glycoside cucumarioside A 2 -2 (CA 2 -2), isolated from the Far Eastern sea cucumber Cucumaria japonica, was compared with lipopolysaccharide (LPS) on mouse spleen. It has been shown that the intraperitoneal (i.p.) glycoside administration leads to increased spleen macrophage activating markers iba-1, IL-1β, iNOs, ROS and NO formation, with additional change of macrophage phenotype to M1. The mass spectrometry profiles of peptide/protein were obtained using MALDI-TOF-MS on the different parts of spleen sections isolated by laser mircodissection techniques. It was found that i.p. stimulation of animals with CA 2 -2 leads to marked changes in the intensity of the characteristic peaks of spleen peptides/proteins, primarily in red pulp. ",

keywords = "IL-1β, Immunomodulation, LPS, NO, ROS, Sea cucumbers, Spleen, Triterpene glycosides, iNOs, iba-1",

author = "Pislyagin, {Evgeny A.} and Manzhulo, {Igor V.} and Gorpenchenko, {Tatiana Y.} and Dmitrenok, {Pavel S.} and Avilov, {Sergey A.} and Silchenko, {Alexandra S.} and Yun-Ming Wang and Aminin, {Dmitry L.}",

year = "2017",

month = nov,

day = "1",

doi = "10.3390/md15110341",

language = "English",

volume = "15",

journal = "Marine Drugs",

issn = "1660-3397",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "11",

}

TY - JOUR

T1 - Cucumarioside A 2 -2 causes macrophage activation in mouse spleen

AU - Pislyagin, Evgeny A.

AU - Manzhulo, Igor V.

AU - Gorpenchenko, Tatiana Y.

AU - Dmitrenok, Pavel S.

AU - Avilov, Sergey A.

AU - Silchenko, Alexandra S.

AU - Wang, Yun-Ming

AU - Aminin, Dmitry L.

PY - 2017/11/1

Y1 - 2017/11/1

N2 - The immunomodulatory effect of triterpene glycoside cucumarioside A 2 -2 (CA 2 -2), isolated from the Far Eastern sea cucumber Cucumaria japonica, was compared with lipopolysaccharide (LPS) on mouse spleen. It has been shown that the intraperitoneal (i.p.) glycoside administration leads to increased spleen macrophage activating markers iba-1, IL-1β, iNOs, ROS and NO formation, with additional change of macrophage phenotype to M1. The mass spectrometry profiles of peptide/protein were obtained using MALDI-TOF-MS on the different parts of spleen sections isolated by laser mircodissection techniques. It was found that i.p. stimulation of animals with CA 2 -2 leads to marked changes in the intensity of the characteristic peaks of spleen peptides/proteins, primarily in red pulp.

AB - The immunomodulatory effect of triterpene glycoside cucumarioside A 2 -2 (CA 2 -2), isolated from the Far Eastern sea cucumber Cucumaria japonica, was compared with lipopolysaccharide (LPS) on mouse spleen. It has been shown that the intraperitoneal (i.p.) glycoside administration leads to increased spleen macrophage activating markers iba-1, IL-1β, iNOs, ROS and NO formation, with additional change of macrophage phenotype to M1. The mass spectrometry profiles of peptide/protein were obtained using MALDI-TOF-MS on the different parts of spleen sections isolated by laser mircodissection techniques. It was found that i.p. stimulation of animals with CA 2 -2 leads to marked changes in the intensity of the characteristic peaks of spleen peptides/proteins, primarily in red pulp.

KW - IL-1β

KW - Immunomodulation

KW - LPS

KW - NO

KW - ROS

KW - Sea cucumbers

KW - Spleen

KW - Triterpene glycosides

KW - iNOs

KW - iba-1

UR - http://www.scopus.com/inward/record.url?scp=85036585214&partnerID=8YFLogxK

U2 - 10.3390/md15110341

DO - 10.3390/md15110341

M3 - Article

C2 - 29104230

AN - SCOPUS:85036585214

SN - 1660-3397

VL - 15

JO - Marine Drugs

JF - Marine Drugs

IS - 11

M1 - 341

ER -

Cucumarioside A ₂ -2 causes macrophage activation in mouse spleen

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this