Coumarin-based Hg 2+ fluorescent probe: Fluorescence turn-on detection for Hg                         2+                          bioimaging in living cells and zebrafish

Chong Guang Chen; Natarajan Vijay; Natesan Thirumalaivasan; Sivan Velmathi; Shu-Pao Wu

doi:10.1016/j.saa.2019.04.048

Coumarin-based Hg ²⁺ fluorescent probe: Fluorescence turn-on detection for Hg ²⁺ bioimaging in living cells and zebrafish

Chong Guang Chen, Natarajan Vijay, Natesan Thirumalaivasan, Sivan Velmathi^*, Shu-Pao Wu

^*Corresponding author for this work

Department of Applied Chemistry

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

The need in developing fluorescent probes for trace metal ion detection in biological samples has been an important issue. Herein, a reaction-based fluorescent probe PIC containing a perimidine moiety was designed and synthesized for Hg ²⁺ detection. The probe can selectively distinguish Hg ²⁺ with 42-fold fluorescent enhancement from the other metal ions at physiological pH. This probe can detect Hg ²⁺ with the detection limit of 1.08 μM. The sensor PIC can be applied to real-time detection of Hg ²⁺ in cells with blue emission.

Original language	English
Pages (from-to)	135-140
Number of pages	6
Journal	Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume	219
DOIs	https://doi.org/10.1016/j.saa.2019.04.048
State	Published - 5 Aug 2019

Keywords

Bioimaging
Coumarin
Hg(II)
Sensor
Zebrafish

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10.1016/j.saa.2019.04.048

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Chen, C. G., Vijay, N., Thirumalaivasan, N., Velmathi, S., & Wu, S.-P. (2019). Coumarin-based Hg ²⁺ fluorescent probe: Fluorescence turn-on detection for Hg ²⁺ bioimaging in living cells and zebrafish. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 219, 135-140. https://doi.org/10.1016/j.saa.2019.04.048

@article{81070c3cfaba4bcf8151bd77444ec418,

title = "Coumarin-based Hg 2+ fluorescent probe: Fluorescence turn-on detection for Hg 2+ bioimaging in living cells and zebrafish",

abstract = " The need in developing fluorescent probes for trace metal ion detection in biological samples has been an important issue. Herein, a reaction-based fluorescent probe PIC containing a perimidine moiety was designed and synthesized for Hg 2+ detection. The probe can selectively distinguish Hg 2+ with 42-fold fluorescent enhancement from the other metal ions at physiological pH. This probe can detect Hg 2+ with the detection limit of 1.08 μM. The sensor PIC can be applied to real-time detection of Hg 2+ in cells with blue emission.",

keywords = "Bioimaging, Coumarin, Hg(II), Sensor, Zebrafish",

author = "Chen, {Chong Guang} and Natarajan Vijay and Natesan Thirumalaivasan and Sivan Velmathi and Shu-Pao Wu",

note = "Publisher Copyright: {\textcopyright} 2019",

year = "2019",

month = aug,

day = "5",

doi = "10.1016/j.saa.2019.04.048",

language = "English",

volume = "219",

pages = "135--140",

journal = "Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy",

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Chen, CG, Vijay, N, Thirumalaivasan, N, Velmathi, S & Wu, S-P 2019, 'Coumarin-based Hg ²⁺ fluorescent probe: Fluorescence turn-on detection for Hg ²⁺ bioimaging in living cells and zebrafish', Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, vol. 219, pp. 135-140. https://doi.org/10.1016/j.saa.2019.04.048

Coumarin-based Hg ²⁺ fluorescent probe: Fluorescence turn-on detection for Hg ²⁺ bioimaging in living cells and zebrafish. / Chen, Chong Guang; Vijay, Natarajan; Thirumalaivasan, Natesan et al.
In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Vol. 219, 05.08.2019, p. 135-140.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Coumarin-based Hg 2+ fluorescent probe

T2 - Fluorescence turn-on detection for Hg 2+ bioimaging in living cells and zebrafish

AU - Chen, Chong Guang

AU - Vijay, Natarajan

AU - Thirumalaivasan, Natesan

AU - Velmathi, Sivan

AU - Wu, Shu-Pao

PY - 2019/8/5

Y1 - 2019/8/5

N2 - The need in developing fluorescent probes for trace metal ion detection in biological samples has been an important issue. Herein, a reaction-based fluorescent probe PIC containing a perimidine moiety was designed and synthesized for Hg 2+ detection. The probe can selectively distinguish Hg 2+ with 42-fold fluorescent enhancement from the other metal ions at physiological pH. This probe can detect Hg 2+ with the detection limit of 1.08 μM. The sensor PIC can be applied to real-time detection of Hg 2+ in cells with blue emission.

AB - The need in developing fluorescent probes for trace metal ion detection in biological samples has been an important issue. Herein, a reaction-based fluorescent probe PIC containing a perimidine moiety was designed and synthesized for Hg 2+ detection. The probe can selectively distinguish Hg 2+ with 42-fold fluorescent enhancement from the other metal ions at physiological pH. This probe can detect Hg 2+ with the detection limit of 1.08 μM. The sensor PIC can be applied to real-time detection of Hg 2+ in cells with blue emission.

KW - Bioimaging

KW - Coumarin

KW - Hg(II)

KW - Sensor

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85064625284&partnerID=8YFLogxK

U2 - 10.1016/j.saa.2019.04.048

DO - 10.1016/j.saa.2019.04.048

M3 - Article

C2 - 31030041

AN - SCOPUS:85064625284

SN - 1386-1425

VL - 219

SP - 135

EP - 140

JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

ER -

Coumarin-based Hg ²⁺ fluorescent probe: Fluorescence turn-on detection for Hg ²⁺ bioimaging in living cells and zebrafish

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Keywords

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