Correlation of K-ras codon 12 mutations in human feces and ages of patients with colorectal cancer (CRC)

Chih Cheng Chien, Shu Hung Chen, Chen Chiung Liu, Chia Long Lee, Ruey Neng Yang, Shung Haur Yang, Chi Jung Huang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Colorectal cancer (CRC) is the predominant gastrointestinal malignancy and constitutes a major medical and economic burden worldwide. A thorough understanding of the oncogenes or genes related to tumorigenesis is the key to developing successful therapeutic strategies. Molecular analysis of feces constitutes a potentially potent and noninvasive method for detection of CRC. Using nested reverse transcription-polymerase chain reaction (RT-PCR) and amplified restriction fragment length polymorphism analysis, sloughed cells from the entire length of the colon and rectum were analyzed for expression of activating K-ras codon 12 mutants, which are becoming attractive targets for antisense treatment. K-ras codon 12 mutant sequences were detected in feces of 5% (1/20) of healthy controls, in feces of 41% (12/29) of CRC patients, in 10% (3/29) of isolates of tissue complementary DNA (cDNA), and in 14% (4/29) of isolates of genomic DNA. Age of patient was significantly associated with K-ras codon 12 sequences in feces: Patients with wild-type K-ras codon 12 sequences were significantly younger than those with mutated forms of K-ras codon 12. Fecal ribonucleic acid (RNA) analysis was demonstrated to be a useful for diagnosis of CRC. This technique may be suitable for screening and determinatign the clinical significance of active mutations of the K-ras gene in feces and would possibly be useful for identificating patients that would benefit from antisense therapy.

Original languageEnglish
Pages (from-to)96-102
Number of pages7
JournalTranslational Research
Volume149
Issue number2
DOIs
StatePublished - Feb 2007

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