Comparative Studies of the Biosynthetic Gene Clusters for Anthraquinone-Fused Enediynes Shedding Light into the Tailoring Steps of Tiancimycin Biosynthesis

Xiaohui Yan, Jian Jun Chen, Ajeeth Adhikari, Christiana N. Teijaro, Huiming Ge, Ivana Crnovcic, Chin-Yuan Chang, Thibault Annaval, Dong Yang, Christoph Rader, Ben Shen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

Comparative analyses of the four known anthraquinone-fused enediynes biosynthetic gene clusters identified four genes, tnmE6, tnmH, tnmL, and tnmQ, unique to the tnm gene cluster. Larger scale fermentation of both the S. sp. CB03234 wild-type and the δtnmH and δtnmL mutant strains resulted in the characterization of 20 new tiancimycin (TNM) congeners, including five enediynes. These findings enabled a proposal for the late stage of TNM biosynthesis featuring an intermediate possibly common for all anthraquinone-fused enediynes.

Original languageEnglish
Pages (from-to)5918-5921
Number of pages4
JournalOrganic Letters
Volume20
Issue number18
DOIs
StatePublished - 21 Sep 2018

Fingerprint

Dive into the research topics of 'Comparative Studies of the Biosynthetic Gene Clusters for Anthraquinone-Fused Enediynes Shedding Light into the Tailoring Steps of Tiancimycin Biosynthesis'. Together they form a unique fingerprint.

Cite this