Comparative hybridization of an array of 21 500 ovarian cDNAs for the discovery of genes overexpressed in ovarian carcinomas

Michèl Schummer; Wai Lap V. Ng; Roger E. Bumgarner; Peter S. Nelson; Bernhard Schummer; David W. Bednarski; Laurie Hassell; Rae Lynn Baldwin; Beth Y. Karlan; Leroy Hood

doi:10.1016/S0378-1119(99)00342-X

Comparative hybridization of an array of 21 500 ovarian cDNAs for the discovery of genes overexpressed in ovarian carcinomas

Michèl Schummer^*, Wai Lap V. Ng, Roger E. Bumgarner, Peter S. Nelson, Bernhard Schummer, David W. Bednarski, Laurie Hassell, Rae Lynn Baldwin, Beth Y. Karlan, Leroy Hood

^*Corresponding author for this work

Department of Biotechnology and Laboratory Science in Medicine

Research output: Contribution to journal › Article › peer-review

346 Scopus citations

Abstract

Comparative hybridization of cDNA arrays is a powerful tool for the measurement of differences in gene expression between two or more tissues. We optimized this technique and employed it to discover genes with potential for the diagnosis of ovarian cancer. This cancer is rarely identified in time for a good prognosis after diagnosis. An array of 21,500 unknown ovarian cDNAs was hybridized with labeled first-strand cDNA from 10 ovarian tumors and six normal tissues. One hundred and thirty-four clones are overexpressed in at least five of the 10 tumors. These cDNAs were sequenced and compared to public sequence databases. One of these, the gene HE4, was found to be expressed primarily in some ovarian cancers, and is thus a potential marker of ovarian carcinoma. (C) 1999 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	375-385
Number of pages	11
Journal	Gene
Volume	238
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1119(99)00342-X
State	Published - 1 Oct 1999

Keywords

Cancer maker
Differential expression
DNA array
HE4

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0378-1119(99)00342-X

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title = "Comparative hybridization of an array of 21 500 ovarian cDNAs for the discovery of genes overexpressed in ovarian carcinomas",

abstract = "Comparative hybridization of cDNA arrays is a powerful tool for the measurement of differences in gene expression between two or more tissues. We optimized this technique and employed it to discover genes with potential for the diagnosis of ovarian cancer. This cancer is rarely identified in time for a good prognosis after diagnosis. An array of 21,500 unknown ovarian cDNAs was hybridized with labeled first-strand cDNA from 10 ovarian tumors and six normal tissues. One hundred and thirty-four clones are overexpressed in at least five of the 10 tumors. These cDNAs were sequenced and compared to public sequence databases. One of these, the gene HE4, was found to be expressed primarily in some ovarian cancers, and is thus a potential marker of ovarian carcinoma. (C) 1999 Elsevier Science B.V. All rights reserved.",

keywords = "Cancer maker, Differential expression, DNA array, HE4",

author = "Mich{\`e}l Schummer and Ng, {Wai Lap V.} and Bumgarner, {Roger E.} and Nelson, {Peter S.} and Bernhard Schummer and Bednarski, {David W.} and Laurie Hassell and Baldwin, {Rae Lynn} and Karlan, {Beth Y.} and Leroy Hood",

note = "Funding Information: This work was supported by the Stowers Institute and by grants from the Deutsche Forschungsgemeinschaft, the National Institutes of Health (5RO1HG01713-02) the Marsha Rivkin Center for Ovarian Cancer Research and the National Science Foundation (BIR9214821/9423347). We would like to thank the members of the sequencing group in our laboratory.",

year = "1999",

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doi = "10.1016/S0378-1119(99)00342-X",

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T1 - Comparative hybridization of an array of 21 500 ovarian cDNAs for the discovery of genes overexpressed in ovarian carcinomas

AU - Schummer, Michèl

AU - Ng, Wai Lap V.

AU - Bumgarner, Roger E.

AU - Nelson, Peter S.

AU - Schummer, Bernhard

AU - Bednarski, David W.

AU - Hassell, Laurie

AU - Baldwin, Rae Lynn

AU - Karlan, Beth Y.

AU - Hood, Leroy

N1 - Funding Information: This work was supported by the Stowers Institute and by grants from the Deutsche Forschungsgemeinschaft, the National Institutes of Health (5RO1HG01713-02) the Marsha Rivkin Center for Ovarian Cancer Research and the National Science Foundation (BIR9214821/9423347). We would like to thank the members of the sequencing group in our laboratory.

PY - 1999/10/1

Y1 - 1999/10/1

N2 - Comparative hybridization of cDNA arrays is a powerful tool for the measurement of differences in gene expression between two or more tissues. We optimized this technique and employed it to discover genes with potential for the diagnosis of ovarian cancer. This cancer is rarely identified in time for a good prognosis after diagnosis. An array of 21,500 unknown ovarian cDNAs was hybridized with labeled first-strand cDNA from 10 ovarian tumors and six normal tissues. One hundred and thirty-four clones are overexpressed in at least five of the 10 tumors. These cDNAs were sequenced and compared to public sequence databases. One of these, the gene HE4, was found to be expressed primarily in some ovarian cancers, and is thus a potential marker of ovarian carcinoma. (C) 1999 Elsevier Science B.V. All rights reserved.

AB - Comparative hybridization of cDNA arrays is a powerful tool for the measurement of differences in gene expression between two or more tissues. We optimized this technique and employed it to discover genes with potential for the diagnosis of ovarian cancer. This cancer is rarely identified in time for a good prognosis after diagnosis. An array of 21,500 unknown ovarian cDNAs was hybridized with labeled first-strand cDNA from 10 ovarian tumors and six normal tissues. One hundred and thirty-four clones are overexpressed in at least five of the 10 tumors. These cDNAs were sequenced and compared to public sequence databases. One of these, the gene HE4, was found to be expressed primarily in some ovarian cancers, and is thus a potential marker of ovarian carcinoma. (C) 1999 Elsevier Science B.V. All rights reserved.

KW - Cancer maker

KW - Differential expression

KW - DNA array

KW - HE4

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DO - 10.1016/S0378-1119(99)00342-X

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SN - 0378-1119

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JO - Gene

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IS - 2

ER -

Comparative hybridization of an array of 21 500 ovarian cDNAs for the discovery of genes overexpressed in ovarian carcinomas

Abstract

Keywords

UN SDGs

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