Comparative genome analysis of Vibrio vulnificus, a marine pathogen

Chung Yung Chen; Keh Ming Wu; Yo Cheng Chang; Chuan Hsiung Chang; Hui Chi Tsai; Tsai Lien Liao; Yen Ming Liu; Hsiang Ju Chen; Arthur Bo Ting Shen; Jian Chiuan Li; Teh Li Su; Chung Ping Shao; Chung Te Lee; Lien I. Hor; Shih Feng Tsai

doi:10.1101/gr.1295503

Comparative genome analysis of Vibrio vulnificus, a marine pathogen

Chung Yung Chen, Keh Ming Wu, Yo Cheng Chang, Chuan Hsiung Chang, Hui Chi Tsai, Tsai Lien Liao, Yen Ming Liu, Hsiang Ju Chen, Arthur Bo Ting Shen, Jian Chiuan Li, Teh Li Su, Chung Ping Shao, Chung Te Lee, Lien I. Hor, Shih Feng Tsai^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

319 Scopus citations

Abstract

The halophile Vibrio vulnificus is an etiologic agent of human mortality from seafood-borne infections. We applied whole-genome sequencing and comparative analysis to investigate the evolution of this pathogen. The genome of biotype 1 strain, V. vulnificus YJ016, was sequenced and includes two chromosomes of estimated 3377 kbp and 1857 kbp in size, and a plasmid of 48,508 bp. A super-integron (SI) was identified, and the SI region spans 139 kbp and contains 188 gene cassettes. In contrast to non-SI sequences, the captured gene cassettes are unique for any given Vibrio species and are highly variable among V. vulnificus strains. Multiple rearrangements were found when comparing the 5.3-Mbp V. vulnificus YJ016 genome and the 4.0-Mbp V. cholerae El Tor N16961 genome. The organization of gene clusters of capsular polysaccharide, iron metabolism, and RTX toxin showed distinct genetic features of V. vulnificus and V. cholerae. The content of the V. vulnificus genome contained gene duplications and evidence of horizontal transfer, allowing for genetic diversity and function in the marine environment. The genomic information obtained in this study can be applied to monitoring vibrio infections and identifying virulence genes in V. vulnificus.

Original language	English
Pages (from-to)	2577-2587
Number of pages	11
Journal	Genome Research
Volume	13
Issue number	12
DOIs	https://doi.org/10.1101/gr.1295503
State	Published - Dec 2003

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1101/gr.1295503

Cite this

@article{8b1346745ec24ac9abcbd2309f294cf2,

title = "Comparative genome analysis of Vibrio vulnificus, a marine pathogen",

abstract = "The halophile Vibrio vulnificus is an etiologic agent of human mortality from seafood-borne infections. We applied whole-genome sequencing and comparative analysis to investigate the evolution of this pathogen. The genome of biotype 1 strain, V. vulnificus YJ016, was sequenced and includes two chromosomes of estimated 3377 kbp and 1857 kbp in size, and a plasmid of 48,508 bp. A super-integron (SI) was identified, and the SI region spans 139 kbp and contains 188 gene cassettes. In contrast to non-SI sequences, the captured gene cassettes are unique for any given Vibrio species and are highly variable among V. vulnificus strains. Multiple rearrangements were found when comparing the 5.3-Mbp V. vulnificus YJ016 genome and the 4.0-Mbp V. cholerae El Tor N16961 genome. The organization of gene clusters of capsular polysaccharide, iron metabolism, and RTX toxin showed distinct genetic features of V. vulnificus and V. cholerae. The content of the V. vulnificus genome contained gene duplications and evidence of horizontal transfer, allowing for genetic diversity and function in the marine environment. The genomic information obtained in this study can be applied to monitoring vibrio infections and identifying virulence genes in V. vulnificus.",

author = "Chen, {Chung Yung} and Wu, {Keh Ming} and Chang, {Yo Cheng} and Chang, {Chuan Hsiung} and Tsai, {Hui Chi} and Liao, {Tsai Lien} and Liu, {Yen Ming} and Chen, {Hsiang Ju} and Shen, {Arthur Bo Ting} and Li, {Jian Chiuan} and Su, {Teh Li} and Shao, {Chung Ping} and Lee, {Chung Te} and Hor, {Lien I.} and Tsai, {Shih Feng}",

year = "2003",

month = dec,

doi = "10.1101/gr.1295503",

language = "English",

volume = "13",

pages = "2577--2587",

journal = "Genome Research",

issn = "1088-9051",

publisher = "Cold Spring Harbor Laboratory Press",

number = "12",

}

TY - JOUR

T1 - Comparative genome analysis of Vibrio vulnificus, a marine pathogen

AU - Chen, Chung Yung

AU - Wu, Keh Ming

AU - Chang, Yo Cheng

AU - Chang, Chuan Hsiung

AU - Tsai, Hui Chi

AU - Liao, Tsai Lien

AU - Liu, Yen Ming

AU - Chen, Hsiang Ju

AU - Shen, Arthur Bo Ting

AU - Li, Jian Chiuan

AU - Su, Teh Li

AU - Shao, Chung Ping

AU - Lee, Chung Te

AU - Hor, Lien I.

AU - Tsai, Shih Feng

PY - 2003/12

Y1 - 2003/12

N2 - The halophile Vibrio vulnificus is an etiologic agent of human mortality from seafood-borne infections. We applied whole-genome sequencing and comparative analysis to investigate the evolution of this pathogen. The genome of biotype 1 strain, V. vulnificus YJ016, was sequenced and includes two chromosomes of estimated 3377 kbp and 1857 kbp in size, and a plasmid of 48,508 bp. A super-integron (SI) was identified, and the SI region spans 139 kbp and contains 188 gene cassettes. In contrast to non-SI sequences, the captured gene cassettes are unique for any given Vibrio species and are highly variable among V. vulnificus strains. Multiple rearrangements were found when comparing the 5.3-Mbp V. vulnificus YJ016 genome and the 4.0-Mbp V. cholerae El Tor N16961 genome. The organization of gene clusters of capsular polysaccharide, iron metabolism, and RTX toxin showed distinct genetic features of V. vulnificus and V. cholerae. The content of the V. vulnificus genome contained gene duplications and evidence of horizontal transfer, allowing for genetic diversity and function in the marine environment. The genomic information obtained in this study can be applied to monitoring vibrio infections and identifying virulence genes in V. vulnificus.

AB - The halophile Vibrio vulnificus is an etiologic agent of human mortality from seafood-borne infections. We applied whole-genome sequencing and comparative analysis to investigate the evolution of this pathogen. The genome of biotype 1 strain, V. vulnificus YJ016, was sequenced and includes two chromosomes of estimated 3377 kbp and 1857 kbp in size, and a plasmid of 48,508 bp. A super-integron (SI) was identified, and the SI region spans 139 kbp and contains 188 gene cassettes. In contrast to non-SI sequences, the captured gene cassettes are unique for any given Vibrio species and are highly variable among V. vulnificus strains. Multiple rearrangements were found when comparing the 5.3-Mbp V. vulnificus YJ016 genome and the 4.0-Mbp V. cholerae El Tor N16961 genome. The organization of gene clusters of capsular polysaccharide, iron metabolism, and RTX toxin showed distinct genetic features of V. vulnificus and V. cholerae. The content of the V. vulnificus genome contained gene duplications and evidence of horizontal transfer, allowing for genetic diversity and function in the marine environment. The genomic information obtained in this study can be applied to monitoring vibrio infections and identifying virulence genes in V. vulnificus.

UR - http://www.scopus.com/inward/record.url?scp=10744231052&partnerID=8YFLogxK

U2 - 10.1101/gr.1295503

DO - 10.1101/gr.1295503

M3 - Article

C2 - 14656965

AN - SCOPUS:10744231052

SN - 1088-9051

VL - 13

SP - 2577

EP - 2587

JO - Genome Research

JF - Genome Research

IS - 12

ER -

Comparative genome analysis of Vibrio vulnificus, a marine pathogen

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this