Clinicopathological significance of MMP-2 and TIMP-2 genotypes in gastric cancer

Chun Ying Wu; Ming Shiang Wu; Yi Ju Chen; Chien Jen Chen; Hsiao Ping Chen; Chia Tung Shun; Gran Hum Chen; Shih Pei Huang; Jaw Town Lin

doi:10.1016/j.ejca.2006.10.022

Clinicopathological significance of MMP-2 and TIMP-2 genotypes in gastric cancer

Chun Ying Wu
, Ming Shiang Wu
, Yi Ju Chen
, Chien Jen Chen
, Hsiao Ping Chen
, Chia Tung Shun
, Gran Hum Chen
, Shih Pei Huang
, Jaw Town Lin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Aims: Single nucleotide polymorphisms in matrix metalloproteinase-2 (MMP-2) -1306 C/T and tissue inhibitor of metalloproteinase-2 (TIMP-2) -418 G/C abolish the Sp-1 binding site and down-regulate expression of these genes. We aim to elucidate the role of MMP-2 and TIMP-2 in clinicopathological manifestations of gastric cancer. Methods: We enrolled 240 gastric cancer patients and 283 controls. DNA was extracted from peripheral blood leucocytes. MMP-2 and TIMP-2 genotypes were analysed by PCR-direct sequencing and PCR-RFLP method, respectively. Results: MMP-2 and TIMP-2 genotypes were not associated with gastric cancer development. However, patients with MMP-2 -1306 C/C genotype showed higher risk of lymphatic invasion (odds ratio (OR) = 2.77, p = 0.01) and venous invasion (OR = 2.93, p = 0.012). TIMP-2 G/G genotype was associated with serosal invasion (OR = 1.89, p = 0.009), lymph node metastasis (OR = 2.19, p = 0.021), lymphatic invasion (OR = 2.87, p = 0.016) and venous invasion (OR = 2.65, p = 0.033). Conclusion: Our results suggest MMP-2 and TIMP-2 genotypes play a crucial role in gastric cancer invasion, but not with development of gastric cancer.

Original language	English
Pages (from-to)	799-808
Number of pages	10
Journal	European Journal of Cancer
Volume	43
Issue number	4
DOIs	https://doi.org/10.1016/j.ejca.2006.10.022
State	Published - Mar 2007

Keywords

Gastric cancer
Invasion
MMP-2
Polymorphism
TIMP-2

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ejca.2006.10.022

Cite this

@article{bc1af893d8dd4cb1b384ae169c29d8b8,

title = "Clinicopathological significance of MMP-2 and TIMP-2 genotypes in gastric cancer",

abstract = "Aims: Single nucleotide polymorphisms in matrix metalloproteinase-2 (MMP-2) -1306 C/T and tissue inhibitor of metalloproteinase-2 (TIMP-2) -418 G/C abolish the Sp-1 binding site and down-regulate expression of these genes. We aim to elucidate the role of MMP-2 and TIMP-2 in clinicopathological manifestations of gastric cancer. Methods: We enrolled 240 gastric cancer patients and 283 controls. DNA was extracted from peripheral blood leucocytes. MMP-2 and TIMP-2 genotypes were analysed by PCR-direct sequencing and PCR-RFLP method, respectively. Results: MMP-2 and TIMP-2 genotypes were not associated with gastric cancer development. However, patients with MMP-2 -1306 C/C genotype showed higher risk of lymphatic invasion (odds ratio (OR) = 2.77, p = 0.01) and venous invasion (OR = 2.93, p = 0.012). TIMP-2 G/G genotype was associated with serosal invasion (OR = 1.89, p = 0.009), lymph node metastasis (OR = 2.19, p = 0.021), lymphatic invasion (OR = 2.87, p = 0.016) and venous invasion (OR = 2.65, p = 0.033). Conclusion: Our results suggest MMP-2 and TIMP-2 genotypes play a crucial role in gastric cancer invasion, but not with development of gastric cancer.",

keywords = "Gastric cancer, Invasion, MMP-2, Polymorphism, TIMP-2",

author = "Wu, \{Chun Ying\} and Wu, \{Ming Shiang\} and Chen, \{Yi Ju\} and Chen, \{Chien Jen\} and Chen, \{Hsiao Ping\} and Shun, \{Chia Tung\} and Chen, \{Gran Hum\} and Huang, \{Shih Pei\} and Lin, \{Jaw Town\}",

note = "Funding Information: This work was supported by the National Science Council, Executive Yuan, Taipei, Taiwan (Grant number: NSC-94-2314-B-075A-017).",

year = "2007",

month = mar,

doi = "10.1016/j.ejca.2006.10.022",

language = "English",

volume = "43",

pages = "799--808",

journal = "European Journal of Cancer",

issn = "0959-8049",

publisher = "Elsevier Ltd",

number = "4",

}

TY - JOUR

T1 - Clinicopathological significance of MMP-2 and TIMP-2 genotypes in gastric cancer

AU - Wu, Chun Ying

AU - Wu, Ming Shiang

AU - Chen, Yi Ju

AU - Chen, Chien Jen

AU - Chen, Hsiao Ping

AU - Shun, Chia Tung

AU - Chen, Gran Hum

AU - Huang, Shih Pei

AU - Lin, Jaw Town

N1 - Funding Information: This work was supported by the National Science Council, Executive Yuan, Taipei, Taiwan (Grant number: NSC-94-2314-B-075A-017).

PY - 2007/3

Y1 - 2007/3

N2 - Aims: Single nucleotide polymorphisms in matrix metalloproteinase-2 (MMP-2) -1306 C/T and tissue inhibitor of metalloproteinase-2 (TIMP-2) -418 G/C abolish the Sp-1 binding site and down-regulate expression of these genes. We aim to elucidate the role of MMP-2 and TIMP-2 in clinicopathological manifestations of gastric cancer. Methods: We enrolled 240 gastric cancer patients and 283 controls. DNA was extracted from peripheral blood leucocytes. MMP-2 and TIMP-2 genotypes were analysed by PCR-direct sequencing and PCR-RFLP method, respectively. Results: MMP-2 and TIMP-2 genotypes were not associated with gastric cancer development. However, patients with MMP-2 -1306 C/C genotype showed higher risk of lymphatic invasion (odds ratio (OR) = 2.77, p = 0.01) and venous invasion (OR = 2.93, p = 0.012). TIMP-2 G/G genotype was associated with serosal invasion (OR = 1.89, p = 0.009), lymph node metastasis (OR = 2.19, p = 0.021), lymphatic invasion (OR = 2.87, p = 0.016) and venous invasion (OR = 2.65, p = 0.033). Conclusion: Our results suggest MMP-2 and TIMP-2 genotypes play a crucial role in gastric cancer invasion, but not with development of gastric cancer.

AB - Aims: Single nucleotide polymorphisms in matrix metalloproteinase-2 (MMP-2) -1306 C/T and tissue inhibitor of metalloproteinase-2 (TIMP-2) -418 G/C abolish the Sp-1 binding site and down-regulate expression of these genes. We aim to elucidate the role of MMP-2 and TIMP-2 in clinicopathological manifestations of gastric cancer. Methods: We enrolled 240 gastric cancer patients and 283 controls. DNA was extracted from peripheral blood leucocytes. MMP-2 and TIMP-2 genotypes were analysed by PCR-direct sequencing and PCR-RFLP method, respectively. Results: MMP-2 and TIMP-2 genotypes were not associated with gastric cancer development. However, patients with MMP-2 -1306 C/C genotype showed higher risk of lymphatic invasion (odds ratio (OR) = 2.77, p = 0.01) and venous invasion (OR = 2.93, p = 0.012). TIMP-2 G/G genotype was associated with serosal invasion (OR = 1.89, p = 0.009), lymph node metastasis (OR = 2.19, p = 0.021), lymphatic invasion (OR = 2.87, p = 0.016) and venous invasion (OR = 2.65, p = 0.033). Conclusion: Our results suggest MMP-2 and TIMP-2 genotypes play a crucial role in gastric cancer invasion, but not with development of gastric cancer.

KW - Gastric cancer

KW - Invasion

KW - MMP-2

KW - Polymorphism

KW - TIMP-2

UR - https://www.scopus.com/pages/publications/33947636758

U2 - 10.1016/j.ejca.2006.10.022

DO - 10.1016/j.ejca.2006.10.022

M3 - Article

C2 - 17236757

AN - SCOPUS:33947636758

SN - 0959-8049

VL - 43

SP - 799

EP - 808

JO - European Journal of Cancer

JF - European Journal of Cancer

IS - 4

ER -

Clinicopathological significance of MMP-2 and TIMP-2 genotypes in gastric cancer

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this