Clinicopathological significance of MMP-2 and TIMP-2 genotypes in gastric cancer

Chun Ying Wu; Ming Shiang Wu; Yi Ju Chen; Chien Jen Chen; Hsiao Ping Chen; Chia Tung Shun; Gran Hum Chen; Shih Pei Huang; Jaw Town Lin

doi:10.1016/j.ejca.2006.10.022

Clinicopathological significance of MMP-2 and TIMP-2 genotypes in gastric cancer

Chun Ying Wu, Ming Shiang Wu, Yi Ju Chen, Chien Jen Chen, Hsiao Ping Chen, Chia Tung Shun, Gran Hum Chen, Shih Pei Huang, Jaw Town Lin^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Aims: Single nucleotide polymorphisms in matrix metalloproteinase-2 (MMP-2) -1306 C/T and tissue inhibitor of metalloproteinase-2 (TIMP-2) -418 G/C abolish the Sp-1 binding site and down-regulate expression of these genes. We aim to elucidate the role of MMP-2 and TIMP-2 in clinicopathological manifestations of gastric cancer. Methods: We enrolled 240 gastric cancer patients and 283 controls. DNA was extracted from peripheral blood leucocytes. MMP-2 and TIMP-2 genotypes were analysed by PCR-direct sequencing and PCR-RFLP method, respectively. Results: MMP-2 and TIMP-2 genotypes were not associated with gastric cancer development. However, patients with MMP-2 -1306 C/C genotype showed higher risk of lymphatic invasion (odds ratio (OR) = 2.77, p = 0.01) and venous invasion (OR = 2.93, p = 0.012). TIMP-2 G/G genotype was associated with serosal invasion (OR = 1.89, p = 0.009), lymph node metastasis (OR = 2.19, p = 0.021), lymphatic invasion (OR = 2.87, p = 0.016) and venous invasion (OR = 2.65, p = 0.033). Conclusion: Our results suggest MMP-2 and TIMP-2 genotypes play a crucial role in gastric cancer invasion, but not with development of gastric cancer.

Original language	English
Pages (from-to)	799-808
Number of pages	10
Journal	European Journal of Cancer
Volume	43
Issue number	4
DOIs	https://doi.org/10.1016/j.ejca.2006.10.022
State	Published - Mar 2007

Keywords

Gastric cancer
Invasion
MMP-2
Polymorphism
TIMP-2

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ejca.2006.10.022

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@article{bc1af893d8dd4cb1b384ae169c29d8b8,

title = "Clinicopathological significance of MMP-2 and TIMP-2 genotypes in gastric cancer",

abstract = "Aims: Single nucleotide polymorphisms in matrix metalloproteinase-2 (MMP-2) -1306 C/T and tissue inhibitor of metalloproteinase-2 (TIMP-2) -418 G/C abolish the Sp-1 binding site and down-regulate expression of these genes. We aim to elucidate the role of MMP-2 and TIMP-2 in clinicopathological manifestations of gastric cancer. Methods: We enrolled 240 gastric cancer patients and 283 controls. DNA was extracted from peripheral blood leucocytes. MMP-2 and TIMP-2 genotypes were analysed by PCR-direct sequencing and PCR-RFLP method, respectively. Results: MMP-2 and TIMP-2 genotypes were not associated with gastric cancer development. However, patients with MMP-2 -1306 C/C genotype showed higher risk of lymphatic invasion (odds ratio (OR) = 2.77, p = 0.01) and venous invasion (OR = 2.93, p = 0.012). TIMP-2 G/G genotype was associated with serosal invasion (OR = 1.89, p = 0.009), lymph node metastasis (OR = 2.19, p = 0.021), lymphatic invasion (OR = 2.87, p = 0.016) and venous invasion (OR = 2.65, p = 0.033). Conclusion: Our results suggest MMP-2 and TIMP-2 genotypes play a crucial role in gastric cancer invasion, but not with development of gastric cancer.",

keywords = "Gastric cancer, Invasion, MMP-2, Polymorphism, TIMP-2",

author = "Wu, {Chun Ying} and Wu, {Ming Shiang} and Chen, {Yi Ju} and Chen, {Chien Jen} and Chen, {Hsiao Ping} and Shun, {Chia Tung} and Chen, {Gran Hum} and Huang, {Shih Pei} and Lin, {Jaw Town}",

note = "Funding Information: This work was supported by the National Science Council, Executive Yuan, Taipei, Taiwan (Grant number: NSC-94-2314-B-075A-017).",

year = "2007",

month = mar,

doi = "10.1016/j.ejca.2006.10.022",

language = "English",

volume = "43",

pages = "799--808",

journal = "European Journal of Cancer",

issn = "0959-8049",

publisher = "Elsevier Ltd",

number = "4",

}

TY - JOUR

T1 - Clinicopathological significance of MMP-2 and TIMP-2 genotypes in gastric cancer

AU - Wu, Chun Ying

AU - Wu, Ming Shiang

AU - Chen, Yi Ju

AU - Chen, Chien Jen

AU - Chen, Hsiao Ping

AU - Shun, Chia Tung

AU - Chen, Gran Hum

AU - Huang, Shih Pei

AU - Lin, Jaw Town

N1 - Funding Information: This work was supported by the National Science Council, Executive Yuan, Taipei, Taiwan (Grant number: NSC-94-2314-B-075A-017).

PY - 2007/3

Y1 - 2007/3

N2 - Aims: Single nucleotide polymorphisms in matrix metalloproteinase-2 (MMP-2) -1306 C/T and tissue inhibitor of metalloproteinase-2 (TIMP-2) -418 G/C abolish the Sp-1 binding site and down-regulate expression of these genes. We aim to elucidate the role of MMP-2 and TIMP-2 in clinicopathological manifestations of gastric cancer. Methods: We enrolled 240 gastric cancer patients and 283 controls. DNA was extracted from peripheral blood leucocytes. MMP-2 and TIMP-2 genotypes were analysed by PCR-direct sequencing and PCR-RFLP method, respectively. Results: MMP-2 and TIMP-2 genotypes were not associated with gastric cancer development. However, patients with MMP-2 -1306 C/C genotype showed higher risk of lymphatic invasion (odds ratio (OR) = 2.77, p = 0.01) and venous invasion (OR = 2.93, p = 0.012). TIMP-2 G/G genotype was associated with serosal invasion (OR = 1.89, p = 0.009), lymph node metastasis (OR = 2.19, p = 0.021), lymphatic invasion (OR = 2.87, p = 0.016) and venous invasion (OR = 2.65, p = 0.033). Conclusion: Our results suggest MMP-2 and TIMP-2 genotypes play a crucial role in gastric cancer invasion, but not with development of gastric cancer.

AB - Aims: Single nucleotide polymorphisms in matrix metalloproteinase-2 (MMP-2) -1306 C/T and tissue inhibitor of metalloproteinase-2 (TIMP-2) -418 G/C abolish the Sp-1 binding site and down-regulate expression of these genes. We aim to elucidate the role of MMP-2 and TIMP-2 in clinicopathological manifestations of gastric cancer. Methods: We enrolled 240 gastric cancer patients and 283 controls. DNA was extracted from peripheral blood leucocytes. MMP-2 and TIMP-2 genotypes were analysed by PCR-direct sequencing and PCR-RFLP method, respectively. Results: MMP-2 and TIMP-2 genotypes were not associated with gastric cancer development. However, patients with MMP-2 -1306 C/C genotype showed higher risk of lymphatic invasion (odds ratio (OR) = 2.77, p = 0.01) and venous invasion (OR = 2.93, p = 0.012). TIMP-2 G/G genotype was associated with serosal invasion (OR = 1.89, p = 0.009), lymph node metastasis (OR = 2.19, p = 0.021), lymphatic invasion (OR = 2.87, p = 0.016) and venous invasion (OR = 2.65, p = 0.033). Conclusion: Our results suggest MMP-2 and TIMP-2 genotypes play a crucial role in gastric cancer invasion, but not with development of gastric cancer.

KW - Gastric cancer

KW - Invasion

KW - MMP-2

KW - Polymorphism

KW - TIMP-2

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U2 - 10.1016/j.ejca.2006.10.022

DO - 10.1016/j.ejca.2006.10.022

M3 - Article

C2 - 17236757

AN - SCOPUS:33947636758

SN - 0959-8049

VL - 43

SP - 799

EP - 808

JO - European Journal of Cancer

JF - European Journal of Cancer

IS - 4

ER -

Clinicopathological significance of MMP-2 and TIMP-2 genotypes in gastric cancer

Abstract

Keywords

UN SDGs

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